Interior circular RNA
Interior circular RNA
复制标题
内部环状RNA
DOI：
10.1080/15476286.2019.1669391
复制
发表时间：
2019-09-28
期刊：
影响因子：
4.1
通讯作者：
Zhang, Weixiong
中科院分区：
文献类型：
--
作者：
Liu, Xiaoxin;Hu, Zhangfeng;Zhang, Weixiong
环状RNA(CircRNAs)是由线状RNA反向剪接或反向融合而成的一类新的真核生物非编码RNA。最近的研究揭示了CircRNAs的剪接体依赖的生物发生，其中CircRNAs出现在mRNAs的内含子-外显子连接处。在这项研究中，我们使用一种新的从头识别方法，证明了CircRNAs可以起源于人、小鼠和水稻的外显子、内含子和基因间转录本的内部区域，称为内部CircRNAs(I-CircRNAs)。许多I-CircRNAs有一些显著的特点：多个I-CircRNAs可能来自同一个基因组位置；它们的后融合点可能与AG/GT剪接点无关，而是一对新的AC/CT基序，它们的后融合点与互补序列相邻；它们可能在短的同源序列上循环。我们通过聚合酶链式反应和Sanger测序验证了HeLa细胞中的几个I-CircRNA。我们的结果综合表明I-CircRNAs是真正的CircRNAs，指示了不依赖于剪接装置的新的生物发生途径，并扩大了我们对CircRNAs的起源、多样性和复杂性的理解。
Formed by back splicing or back fusion of linear RNAs, circular RNAs (circRNAs) constitute a new class of non-coding RNAs of eukaryotes. Recent studies reveal a spliceosome-dependent biogenesis of circRNAs where circRNAs arise at the intron-exon junctions of mRNAs. In this study, using a novel de novo identification method, we show that circRNAs can originate from the interior regions of exons, introns, and intergenic transcripts in human, mouse and rice, which were referred to as interior circRNAs (i-circRNAs). Many i-circRNAs have some remarkable characteristics: multiple i-circRNAs may arise from the same genomic locus; their back fusion points may not be associated with the AG/GT splicing sites, but rather a new pair of motif AC/CT, their back fusion points are adjacent to complementary sequences; and they may circulate on short homologous sequences. We validated several i-circRNAs in HeLa cells by Polymerase Chain Reaction followed by Sanger sequencing. Our results combined showed that i-circRNAs are bona fide circRNAs, indicated novel biogenesis pathways independent of the splicing apparatus, and expanded our understanding of the origin, diversity, and complexity of circRNAs.