Chromosomal Location of the Structural Gene for Glycerol Kinase in Escherichia coli
Chromosomal Location of the Structural Gene for Glycerol Kinase in Escherichia coli
复制标题
大肠杆菌甘油激酶结构基因的染色体定位
作者：
N. Cozzarelli;E. Lin
Cozzarelli，N. R.（哈佛医学院，波士顿，马萨诸塞州），和E. C. C.是林书大肠杆菌甘油激酶结构基因的染色体定位。J. Bacteriol. 91：1763-1766。1966.-一个甘油激酶突变位点已被映射的转导和性接合。三因子杂交与两个程序产生了以下基因顺序：精氨酸-1-甲硫氨酸-1-甘油激酶-异亮氨酸，缬氨酸-16。另外13个独立的甘油激酶突变位点定位在同一区域。由于一些突变体能够产生一种蛋白质血清学上与野生型酶无法区分，因此可以得出结论，该区域映射代表激酶的结构基因。
Cozzarelli, N. R. (Harvard Medical School, Boston, Mass.), and E. C. C. Lin. Chromosomal location of the structural gene for glycerol kinase in Escherichia coli. J. Bacteriol. 91:1763–1766. 1966.—A glycerol kinase mutant site has been mapped by transduction and sexual conjugation. Three-factor crosses with the two procedures yielded the following gene order: arginine-1–methionine-1–glycerol kinase–isoleucine, valine-16. An additional 13 independent glycerol kinase mutant sites mapped in the same region. Since some of the mutants were able to produce a protein serologically indistinguishable from the wild-type enzyme, it is concluded that the region mapped represents the structural gene for the kinase.