Lymphocyte-filled villi: Comparison with other lymphoid aggregations in the mucosa of the human small intestine
Lymphocyte-filled villi: Comparison with other lymphoid aggregations in the mucosa of the human small intestine
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DOI：
10.1016/s0016-5085(98)70020-4
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发表时间：
1998-12-01
期刊：
影响因子：
29.4
通讯作者：
Mayrhofer, G
中科院分区：
文献类型：
--
作者：
Moghaddami, M;Cummins, A;Mayrhofer, G
背景和目的：最近在小鼠（隐窝斑）和大鼠（充满淋巴细胞的绒毛）小肠中描述了可能是原发性胸腺外 T 细胞分化位点的孤立淋巴结构，本研究检验了类似结构出现在人类小肠中的假设。方法：手术期间获得正常小肠，对固定组织进行组织学检查，并使用一组小鼠单克隆抗体通过间接免疫过氧化物酶技术对冰冻切片进行检查。结果：一种新的分离的淋巴结构，其上皮类似于派尔氏斑的滤泡相关上皮，被描述为充满淋巴细胞的绒毛，这些结构包含主要组织相容性复合体（MHC）II类阳性树突状细胞，大多数记忆T细胞，可变的B细胞成分，并且没有表达c-kit或CD1a的未成熟淋巴细胞的证据，两个先前描述的淋巴聚集体（分离的淋巴）滤泡和粘膜下淋巴聚集体）是单一结构的组成部分，完整的结构包含 B 细胞滤泡、主要具有记忆（CD45RO 阳性）表型的 T 细胞、高内皮微静脉和未检测到的未成熟淋巴细胞群。结论：在人类小肠中描述了一种新的孤立淋巴结构。这些结构和孤立的淋巴滤泡似乎都与啮齿动物肠道中孤立的首要淋巴结构相似。
Background & Aims: Solitary lymphoid structures that may be sites of primary extrathymic T-cell differentiation have been described recently in murine (crypto-patches) and rat (lymphocyte-filled villi) small intestine, This study tests the hypothesis that similar structures occur in human small intestine. Methods: Normal small intestine was obtained during surgery, Fixed tissue was examined histologically, and frozen sections were examined by an indirect immunoperoxidase technique using a panel of mouse monoclonal antibodies. Results: A new isolated lymphoid structure, with epithelium resembling follicle-associated epithelium of Peyer's patches, is described as a lymphocyte-filled villus, These structures contain major histocompatibility complex (MHC) class Ii-positive dendritic cells, a majority of memory T cells, a variable B-cell component, and no evidence of immature lymphocytes that express either c-kit or CD1a, Two previously described lymphoid aggregations (isolated lymphoid follicles and submucosal lymphoid aggregations) are components of a single structure, The complete structure contains a B-cell follicle, T cells with mainly memory (CD45RO-positive) phenotype, high endothelial venules, and no detectable population of immature lymphocytes, Conclusions: A new solitary lymphoid structure is described in the human small intestine. Neither these structures nor isolated lymphoid follicles appear to be similar to solitary primacy lymphoid structures in rodent intestine.