A PC12 variant lacking regulated secretory organelles: aberrant protein targeting and evidence for a factor inhibiting neuroendocrine gene expression.
A PC12 variant lacking regulated secretory organelles: aberrant protein targeting and evidence for a factor inhibiting neuroendocrine gene expression.
复制标题
缺乏受调节分泌细胞器的 PC12 变体：异常的蛋白质靶向和抑制神经内分泌基因表达的因素的证据。
DOI：
10.1046/j.1471-4159.1999.0730021.x
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发表时间：
1999
影响因子：
4.7
通讯作者：
Jackson,AP
中科院分区：
文献类型：
--
作者：
Pance,A;Morgan,K;Guest,PC;Bowers,K;Dean,GE;Cutler,DF;Jackson,AP
翻译后摘要：PC 12嗜铬细胞瘤细胞系（称为A35 C）的变体已被分离，缺乏调节分泌细胞器和几个组成蛋白。北方和南方印迹分析表明，在转录水平上的块。前蛋白转化酶羧肽酶H在A35 C细胞系中合成，但通过组成型途径分泌。用编码调节分泌蛋白多巴胺β-羟化酶和突触结合蛋白I的cDNA瞬时转染A35 C细胞，导致这些蛋白质的明显错误模式。令人惊讶的是，通过融合正常PC 12细胞与A35 C细胞产生的杂交细胞表现出变异表型，表明A35 C细胞表达抑制神经内分泌特异性基因表达的抑制因子。
Abstract:A variant of the PC12 pheochromocytoma cell line (termed A35C) has been isolated that lacks regulated secretory organelles and several constituent proteins. Northern and Southern blot analyses suggested a block at the transcriptional level. The proprotein‐converting enzyme carboxypeptidase H was synthesised in the A35C cell line but was secreted by the constitutive pathway. Transient transfection of A35C cells with cDNAs encoding the regulated secretory proteins dopamine β‐hydroxylase and synaptotagmin I resulted in distinct patterns of mistargeting of these proteins. It is surprising that hybrid cells created by fusing normal PC12 cells with A35C cells exhibited the variant phenotype, suggesting that A35C cells express an inhibitory factor that represses neuroendocrine‐specific gene expression.