Accumulation of p53 protein in human esophageal precancerous lesions: a possible early biomarker for carcinogenesis.
Accumulation of p53 protein in human esophageal precancerous lesions: a possible early biomarker for carcinogenesis.
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发表时间：
1993-04
期刊：
影响因子：
11.2
通讯作者：
Li Dong Wang;Jun-Yan Hong;S. Qiu;Hongkun Gao;Chung S. Yang
中科院分区：
文献类型：
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作者：
Li Dong Wang;Jun-Yan Hong;S. Qiu;Hongkun Gao;Chung S. Yang
通过免疫组织化学方法测定正常组织和不同严重程度病变组织（基底细胞增生、不典型增生、原位癌和癌）的p53蛋白水平，这些组织来自手术切除的人食管和无症状受试者的食管活检。样本来自中国北方食管癌高发地区（河南省临县和辉县）。将组织切片与 p53 抗体一起孵育以进行免疫染色。还使用常规的苏木精和伊红染色。在手术切除的食管标本中，在癌前病变和癌病变组织的细胞核中发现 p53 蛋白水平升高。从基底细胞增生到不典型增生再到原位癌，p53免疫染色阳性细胞数量增多，其分布与增殖细胞大致相同。然而，在手术切除的组织的正常上皮的分裂基底细胞中未观察到阳性染色。在无症状受试者的活检样本的异常组织中观察到类似的免疫染色模式。一个有趣的观察是，在活检样本的 6 例组织学正常上皮细胞中，有 3 例观察到一些 p53 免疫染色阳性细胞。在这三个“正常”病例中仅观察到乳头状免疫染色模式。尽管这种阳性染色的分子基础仍有待研究，但 p53 蛋白积累可能发生在食管癌发病机制的早期，并且 p53 突变与这种癌症的发生密切相关。 p53 蛋白的积累可能是识别食管癌高危受试者的有前景的早期生物标志物。
The level of p53 protein was determined immunohistochemically in normal tissues and tissues with different severities of lesions (basal cell hyperplasia, dysplasia, carcinoma in situ, and carcinoma) from surgically resected human esophagi and esophageal biopsies of symptom-free subjects. The samples were from an area with high esophageal cancer incidence in northern China (Linxian and Huixian in the Henan province). Tissue sections were incubated with p53 antibodies for immunostaining. Conventional hematoxylin and eosin stain was also used. In surgically resected esophageal specimens, elevated p53 protein levels were found in the cell nuclei in tissues with precancerous and cancerous lesions. From basal cell hyperplasia to dysplasia to carcinoma in situ, the p53 immunostain-positive cells increased in number, and their distribution had roughly the same pattern as that of the proliferating cells. However, positive stain was not observed in the dividing basal cells of the normal epithelium of the surgically resected tissues. A similar pattern of immunostaining was observed in the abnormal tissues of the biopsy samples from the symptom-free subjects. An intriguing observation is that some p53 immunostain-positive cells were observed in 3 of 6 cases of histologically normal epithelia of biopsy samples. Only the papillary immunostaining pattern was observed in these three "normal" cases. Although the molecular basis for such positive stain remains to be investigated, it is possible that p53 protein accumulation occurs early in the pathogenesis of esophageal cancer and that p53 mutation is closely associated with the initiation of this cancer. The accumulation of p53 protein may be a promising early biomarker for identifying high-risk subjects for esophageal cancer.