Isolation and Identification of Achromobacter sp DN-06 and Evaluation of Its Pyridine Degradation Kinetics
Isolation and Identification of Achromobacter sp DN-06 and Evaluation of Its Pyridine Degradation Kinetics
复制标题
无色杆菌DN-06的分离鉴定及其吡啶降解动力学评价
DOI：
10.1007/s11270-011-0796-7
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发表时间：
2011-10-01
影响因子：
2.9
通讯作者：
Chai, Xinsheng
中科院分区：
文献类型：
--
作者：
Deng, Xiuqiong;Wei, Chaohai;Chai, Xinsheng
We report the isolation of a new bacterium species (named as DN-06) that degrades pyridine, a model compound containing both carbon and nitrogen, from the aerobic activated sludge in a coking wastewater treatment plant. DN-06 was identified as Achromobacter sp. using 16S rDNA sequence analysis. In batch culture, more than 95% of pyridine (500 mg/L) was degraded within 18 h by DN-06 grown at 35 degrees C and pH 8 with agitation at 170 rpm. Degradation experiments of pyridine at different initial concentrations (50-4,300 mg/L) revealed that pyridine was an inhibitory substrate, and that neither yield coefficient Y nor endogenous decay coefficient K(d) was a constant. The values of Y and K(d) were 0.55-0.74 and 0.0032-0.0057 h(-1), respectively. Five kinetic models (Haldane, Yano, Aiba, Webb, and Monod) were fitted to the experimental growth kinetic data. Models of Haldane and Yano (correlation coefficient R(2)=0.929) were the most suitable models. For Haldane kinetic model, the values of mu(max), K(s), and K(i) were 0.161 h(-1), 142.6 mg/L, and 4234.8 mg/L, respectively. The large values of Y and Ki indicated that DN-06 had good tolerance against high pyridine concentrations. These results indicated possible future applications of Achromobacter sp. DN-06 in removing pyridine from industrial wastewaters, as well as in destroying pyridine in concentrated solutions during further treatment of trial water coupling with adsorption technology.
