High-performance multiplex microvalves fabrication and using for tumor cells staining on a microfluidic chip
High-performance multiplex microvalves fabrication and using for tumor cells staining on a microfluidic chip
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DOI：
10.1007/s10544-019-0434-5
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发表时间：
2019-12-01
影响因子：
2.8
通讯作者：
Liu, Kan
中科院分区：
文献类型：
--
作者：
Hong, Shao-Li;Tang, Man;Liu, Kan
众所周知，微阀在芯片微流控操作中具有重要的作用。本文报道了一种简单的高性能多路微阀芯片制作方法。在这种方法中，通过将聚二甲基硅氧烷（PDMS）膜插入到两个玻璃中来建立三明治结构，该方法便宜且简单，无需任何复杂的硅基器件或软光刻。利用PDMS的弹性和玻璃的刚性，该微阀芯片具有良好的控制性能和多路集成能力。此外，在计算机设计程序的辅助下，这种微阀芯片可以自动执行，显示出巨大的潜力，开发新的高度集成的微流控器件。此外，所制作的多重微阀芯片进一步成功地用于肿瘤细胞的自动染色，提高了细胞鉴定过程的效率，减少了人为误差。这些结果表明，这种方法开辟了新的途径，多个微阀的制造和其生物应用。
As we all know, microvalve holds great importance for microfluidic manipulation in chip. Herein, a simple method of high-performance multiplex microvalves chip fabrication was reported. In this method, a sandwich structure is established by inserting a polydimethylsiloxane (PDMS) membrane into two glasses, which is cheap and simple without any complex silicon-based device or soft lithography. Taking advantages of both the elasticity of the PDMS and the rigidity of glass, the microvalve chip showed good controls performance and had the ability of multiplex integration. Moreover, aided by a computer design program, this microvalves chip can be performed automatically, showing great potential to develop new highly integrated microfluidic devices. In addition, the fabricated multiplex microvalve chip is further successfully used for staining tumor cells automatically, improving the efficiency of cell identification process and reducing human errors. These results indicate this method opens up new avenues for multiplex microvalves fabrication and its biological application.