Quantitative analyses of cell division in plants
Quantitative analyses of cell division in plants
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DOI：
10.1007/s11103-005-4065-2
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发表时间：
2006-04-01
影响因子：
5.1
通讯作者：
Beemster, Gerrit T. S.
中科院分区：
文献类型：
--
作者：
Fiorani, Fabio;Beemster, Gerrit T. S.
在分子水平上，细胞周期基因之间的调控相互作用正被迅速揭示，但在阐明这些分子事件如何在细胞和整个生物体水平上调控生长过程方面进展较少。主要障碍是缺乏一套分析工具，既要有足够的能力确定相关参数，又要相对容易为非专业实验室所用。在上个世纪的前半叶，已经开创了获得这类数据的适当方法，现在通常被定义为“运动学分析”。不幸的是，这些分析的费力性质和使用的相对复杂的数值方法，限制了它们的使用，只有少数专业研究小组。在这篇文章中，我们试图提出一个可访问的入口，这种方法，特别是在数学框架。我们开始描述可能最简单的系统，即，实质上均质的细胞悬浮培养物。然后，我们概述了双子叶植物的叶，根尖，单子叶植物的叶，最后拍摄顶端分生组织的分析。对于这些系统中的每一个，我们讨论了细胞分裂参数，如细胞周期的持续时间，分生组织的大小和其中包含的细胞的数量的计算的细节，这使得能够回答有关细胞生产和细胞大小的变化在生长中的相对贡献的基本问题。此外，我们还讨论了这些方法和替代方法的假设和局限性，旨在根据具体的研究问题促进适当分析的选择。
At the molecular level regulatory interactions between cell cycle genes are being uncovered rapidly, but less progress is made in unravelling how these molecular events regulate growth processes at the level of cells and of the whole organism. The main obstacle is the absence of a set of analytical tools that are powerful enough to determine pertinent parameters and, at the same time, relatively easy to use by non-specialized laboratories. Appropriate methodology to obtain this type of data has been pioneered in the first half of the last century and is now commonly defined as 'kinematic analysis'. Unfortunately, the laborious nature of these analyses and the relatively complex numerical methods used, have limited their use to only a handful of specialized research groups. In this article we attempt to present an accessible entry to this methodology, particularly in terms of the mathematical framework. We start describing the simplest possible system, i.e., a virtually homogenous cell suspension culture. Then, we outline the analysis of dicotyledonous leaves, root tips, monocotyledonous leaves, and finally shoot apical meristems. For each of these systems we discuss the details of the calculation of cell division parameters such as cell cycle duration, size of the meristem and number of cells contained in it, which enables answering fundamental questions about the relative contribution of differences in cell production and cell size to variation in growth. In addition, we discuss the assumptions and limitations of these and alternative methodologies with the aim to facilitate the choice of appropriate analyses depending on the specific research question.