NEUROFILAMENT AND TUBULIN EXPRESSION RECAPITULATES THE DEVELOPMENTAL PROGRAM DURING AXONAL REGENERATION - INDUCTION OF A SPECIFIC BETA-TUBULIN ISOTYPE
NEUROFILAMENT AND TUBULIN EXPRESSION RECAPITULATES THE DEVELOPMENTAL PROGRAM DURING AXONAL REGENERATION - INDUCTION OF A SPECIFIC BETA-TUBULIN ISOTYPE
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DOI：
10.1073/pnas.85.12.4530
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发表时间：
1988-06-01
影响因子：
11.1
通讯作者：
CLEVELAND, DW
中科院分区：
文献类型：
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作者：
HOFFMAN, PN;CLEVELAND, DW
我们检查了大鼠感觉神经元在发育、成熟和轴突再生过程中编码三种β-微管蛋白同种型（I、II和IV类）和68-kDa神经丝蛋白（NF68）的基因的差异表达。编码II类同种型的特定β-微管蛋白基因的表达在发育和轴突再生期间被诱导至高水平，而编码其他两种同种型(I类和IV类)的基因的表达仍与成熟水平相当。 相反，NF68基因的表达在发育和再生过程中相对较低。因此，细胞骨架基因表达的发育程序在轴突再生过程中得到了概括。在发育中的再生神经元中发现的高水平的II类β-微管蛋白表达发生在轴突的纵向生长期间。相反，NF68 基因表达的诱导与成熟神经元中轴突的径向生长相关。
We examined the differential expression of genes encoding three .beta.-tubulin isotypes (classes I, II, and IV) and the 68-kDa neurofilament protein (NF68) in rat sensory neurons during development, maturation, and axonal regeneration. Expression of the specific .beta.-tubulin gene encoding the class II isotype was induced to high levels during development and axonal regeneration, whereas the expression of genes encoding the two other isotypes (classes I and IV) remained comparable to mature levels. Conversely, expression of the NF68 gene was relatively low during development and regeneration. Thus, the developmental program for cytoskeletal gene expression is recapitulated during axonal regeneration. The high level of class II .beta.-tubulin expression found in developing regenerating neurons occurs during the longitudinal growth of axons. In contrast, induction of NF68 gene expression is associated with the radial growth of axons in maturing neurons.