A microsensor for urea based on an ion-selective field effect transistor
A microsensor for urea based on an ion-selective field effect transistor
复制标题
基于离子选择场效应晶体管的尿素微传感器
DOI：
10.1016/0003-2670(86)80046-0
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
M. Gotoh
中科院分区：
文献类型：
--
作者：
Isao Karbue;E. Tamiya;J. Dicks;M. Gotoh
The pH response of a pH-sensitive field-effect transistor (FET) is not affected by a ca. 1-μm thick membrane formed from λ-aminopropyltriethoxysilane and glutaraldehyde over the gate insular by a vapor deposition method. The response between pH 5.5 and 8.5 is ca. 61 mV pH−1at 37°C in 5 mM Tris-HCl buffer. When urease is immobilized on the membrane, the sensor gives a linear relationship between the initial rate of the output gate voltage change and the logarithmic value of urea concentration between 16.7 and 167 mM. Determination of urea is possible within 30 s. The optimum pH is 6.0–6.5, at 37°C. The system can be used for 20 days with only slight loss of enzymatic activity.
