Complete genome sequence of Sikte (Sitke) waterborne virus, a member of the genus Tombusvirus
Complete genome sequence of Sikte (Sitke) waterborne virus, a member of the genus Tombusvirus
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DOI：
10.1007/s00705-020-04949-0
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发表时间：
2021-01-25
影响因子：
2.7
通讯作者：
Hanada, Kaoru
中科院分区：
文献类型：
--
作者：
Uehara-Ichiki, Tamaki;Urayama, Syun-ichi;Hanada, Kaoru
Sikte水传播病毒（SWV）的4704-nt基因组序列，通过片段化和引物连接的dsRNA测序和直接Sanger测序确定，是线性的，非片段化的，具有其他tombusvirus的5个orf。5 ‘和3 ’未翻译区（utr）分别为150和335 nt长。外壳蛋白的系统发育分析表明，SWV与CymRSV和PNSV相关，而SWV复制酶蛋白p92的系统发育分析表明，SWV与CNV关系密切。这些系统发育分析表明，在SWV中发生了重组事件，就像以前报道的其他tombusvirus一样。
The 4704-nt genome sequence of Sikte waterborne virus (SWV), determined by fragmented and primer ligated dsRNA sequencing and by direct Sanger sequencing, is linear, nonsegmented and has the five ORFs of other tombusviruses. The 5 ' and 3 ' untranslated regions (UTRs) are 150 and 335 nt long, respectively. Phylogenetic analysis of the coat protein revealed that SWV is related to CymRSV and PNSV, but that of the SWV replicase protein, the p92 readthrough protein, indicated a close relationship to CNV. These phylogenetic analyses suggest the occurrence of recombination events in SWV, as reported previously for other tombusviruses.