Variation in Splicing Efficiency Underlies Morphological Evolution in Capsella
Variation in Splicing Efficiency Underlies Morphological Evolution in Capsella
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DOI：
10.1016/j.devcel.2017.11.022
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发表时间：
2018-01-22
影响因子：
11.8
通讯作者：
Lenhard, Michael
中科院分区：
文献类型：
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作者：
Fujikura, Ushio;Jing, Runchun;Lenhard, Michael
了解形态变化的分子基础仍然是进化发育生物学的一个中心挑战。从远缘繁殖到自交的转变往往伴随着生殖结构和功能的急剧减少，例如两性线虫失去了诱人的信息素，植物的花变小了。在这里，我们证明了油菜素类固醇生物合成酶CYP724A1水平的变化导致了自交风信子与其近交祖先格兰迪弗罗拉相比花朵尺寸的减小。风疹弧菌等位基因的初级转录本的剪接效率高于格兰迪弗拉弧菌，导致油菜素类固醇水平较高。它们通过限制细胞增殖来限制器官的生长。更有效的风疹杆菌等位基因剪接来自自交谱系中的两个从头突变。因此，我们的结果强调了差异剪接效率和高于最佳水平的激素水平在产生表型变异方面潜在的广泛重要性。
Understanding the molecular basis of morphological change remains a central challenge in evolutionary-developmental biology. The transition from outbreeding to selfing is often associated with a dramatic reduction in reproductive structures and functions, such as the loss of attractive pheromones in hermaphroditic Caenorhabditis elegans and a reduced flower size in plants. Here, we demonstrate that variation in the level of the brassinosteroid-biosynthesis enzyme CYP724A1 contributes to the reduced flower size of selfing Capsella rubella compared with its outbreeding ancestor Capsella grandiflora. The primary transcript of the C. rubella allele is spliced more efficiently than that of C. grandiflora, resulting in higher brassinosteroid levels. These restrict organ growth by limiting cell proliferation. More efficient splicing of the C. rubella allele results from two de novo mutations in the selfing lineage. Thus, our results highlight the potentially widespread importance of differential splicing efficiency and higher-than-optimal hormone levels in generating phenotypic variation.