A Pilot Study of Uterine Artery Embolization with Tris-Acryl Gelatin Microspheres in Guinea Pigs
A Pilot Study of Uterine Artery Embolization with Tris-Acryl Gelatin Microspheres in Guinea Pigs
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DOI：
10.1007/s00270-011-0215-x
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发表时间：
2012-06
影响因子：
2.9
通讯作者：
Wenquan Zhuang;Guosheng Tan;Wenbo Guo;Jian-yong Yang
中科院分区：
文献类型：
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作者：
Wenquan Zhuang;Guosheng Tan;Wenbo Guo;Jian-yong Yang
目的建立三酰基明胶微球(TAGM)栓塞术(UAE)的动物模型。方法成年雌性豚鼠25只，随机分为子宫动脉铸型组(n=10)和UAE组(n=15)。第一组行盆腔血管造影术和血管铸型。本文报道了豚鼠盆腔的解剖学特征。在第二组中，研究了用TAGM行豚鼠UAE的技术可行性。结果子宫动脉起自髂内动脉，沿宫颈曲折上行，垂直发出8~10支分支至子宫颈和子宫角。子宫动脉主干外径为0.32±0.027 mm，第一支外径为0.14±0.01 mm。对于阿联酋动物，40-120g和100-300gμm TAGM的剂量分别为0.033±0.003 mg/ml和0.015±0.002 mg/ml。在组织病理学切片上，子宫动脉的第一分支、浆膜下动脉和壁内动脉可见卵泡颗粒。炎症反应在UAE后的豚鼠中很常见。少数豚鼠可见子宫局部或弥漫性坏死区。结论豚鼠是UAE合并TAGM的合适、可行的动物模型。
ObjectiveThis study was designed to establish guinea pigs as an animal model for uterine artery embolization (UAE) with tris-acryl gelatin microspheres (TAGM).MethodsTwenty-five female adult guinea pigs were randomly divided into two groups, including a uterine artery casting mould group (n= 10) and a UAE group (n= 15). Pelvic angiography and vascular casting mould were performed in the first group. The anatomical characters of the pelvic cavity in guinea pigs were described. In the second group, the technical feasibility of performing UAE with TAGM in guinea pigs was investigated. The histopathological slides of the uterus of guinea pigs after UAE were examined to inspect the outcomes of UAE.ResultsThe uterine artery springs from the internal iliac artery, ascends tortuously along the cervix, and gives off vertically 8–10 branches to the cervix uteri and uterine horns. The diameters of the trunk of the uterine artery and its first branch were 0.32 ± 0.027 mm and 0.14 ± 0.01 mm, respectively. For UAE animals, the dosages of 40–120 and 100–300 μm TAGM were 0.033 ± 0.003 ml and 0.015 ± 0.002 ml, respectively. On histopathological slides, embosphere particles were found in the first branches of the uterine artery, the subserous arteries, and the intramural arteries. Inflammatory reactions in the uterus were common in guinea pigs after UAE. Local or dispersed areas of necrosis in uterus also were observed in a few guinea pigs.ConclusionsGuinea pigs are an appropriate and feasible model for UAE with TAGM.