Enhancement of adenovirus plaque formation on HeLa cells by magnesium chloride.
Enhancement of adenovirus plaque formation on HeLa cells by magnesium chloride.
复制标题
氯化镁增强 HeLa 细胞上腺病毒噬斑的形成。
DOI：
10.1099/0022-1317-9-3-251
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发表时间：
1970
期刊：
影响因子：
--
通讯作者：
J. Williams
中科院分区：
文献类型：
--
作者：
J. Williams
二价阳离子对哺乳动物细胞对病毒感染的易感性的影响一直是几项研究的主题。钙可增强兔胚肾细胞上脊髓灰质炎病毒空斑的形成（库珀，1961），镁可增加猴肾细胞对脊髓灰质炎病毒的易感性（沃利斯& Melnick，1962）。某些鼻病毒在HeLa细胞中形成的空斑通过覆盖培养基中较高浓度的钙和镁而增强（Fiala & Kenny，1966; Stott & Tyrrell，1968）。猴肾细胞对脊髓灰质炎病毒的易感性增加是由于病毒从受感染细胞中提前释放所致（沃利斯& Melnick，1962），并且在高水平MgCl 2存在下，鼻病毒从HeLa细胞中的释放也大大增强（Fiala & Kenny，1967）。这一通信提供了证据表明，氯化镁也增强了人腺病毒在HeLa细胞单层上的空斑形成，这种效果是由于感染细胞释放病毒的速率增加。
The effect of divalent cations on the susceptibility of mammalian cells to infection by viruses has been the subject of several investigations. Calcium enhances poliovirus plaque formation on rabbit embryo kidney cells (Cooper, 1961) and magnesium increases the susceptibility of monkey kidney cells to poliovirus (Wallis & Melnick, 1962). Plaque formation by certain rhinoviruses in HeLa cells is enhanced by higher concentrations of calcium and magnesium in the overlay medium (Fiala & Kenny, 1966; Stott & Tyrrell, 1968). The increased susceptibility of monkey kidney cells to poliovirus results from the earlier release of virus from infected cells (Wallis & Melnick, 1962), and the release of rhinovirus from HeLa cells is also greatly enhanced in the presence of high levels of MgCl2 (Fiala & Kenny, 1967). This communication provides evidence that MgCl2 also enhances plaque formation by human adenoviruses on HeLa cell monolayers and that the effect is due to an increase in the rate of virus release from infected cells.