Conductive Nerve Guidance Conduits Based on Morpho Butterfly Wings for Peripheral Nerve Repair
Conductive Nerve Guidance Conduits Based on Morpho Butterfly Wings for Peripheral Nerve Repair
复制标题
基于形态蝶翼的周围神经传导导引导管修复周围神经
DOI：
10.1021/acsnano.1c11627
复制
发表时间：
2022-02-22
期刊：
影响因子：
17.1
通讯作者：
Chai, Renjie
中科院分区：
文献类型：
--
作者：
Hu, Yangnan;Chen, Zhuoyue;Chai, Renjie
周围神经损伤（Peripheral nerve injury，PNI）是一种神经再生能力有限的复杂疾病，可导致感觉和运动功能的丧失。神经引导导管（NGC）已成为一种有前途的替代周围神经再生，但其疗效往往是有限的。在这里，受周围神经生理结构的启发，我们提出了一种用于神经修复的导电拓扑支架，通过用还原氧化石墨烯（rGO）纳米片和甲基丙烯酸化明胶（GelMA）水凝胶包裹脑源性神经营养因子（BDNF）修饰Morpho蝴蝶翅膀。受益于GelMA水凝胶的生物相容性，rGO的导电性和翼鳞的平行纳米脊结构，PC 12细胞和在修饰的翼上生长的神经干细胞具有增加的神经突长度和引导的细胞取向。此外，通过手动卷起支架成功获得NGC，并在修复大鼠10 mm坐骨神经缺损中表现出良好的性能，我们相信通过进一步优化，NGC可以在未来应用于修复更长的神经缺损。我们还证明了基于rGO/BDNF/GelMA整合的Morpho蝴蝶翅膀的导电NGC作为功能性神经再生管道的可行性，这可能在修复周围神经损伤中具有潜在的应用价值。
Peripheral nerve injury (PNI), causing loss of sensory and motor function, is a complex and challenging disease in the clinic due to the restricted regeneration capacity. Nerve guidance conduits (NGCs) have become a promising substitute for peripheral nerve regeneration, but their efficacy is often limited. Here, inspired by the physiological structures of peripheral nerves, we present a conductive topological scaffold for nerve repair by modifying Morpho butterfly wing with reduced graphene oxide (rGO) nanosheets and methacrylated gelatin (GelMA) hydrogel encapsulated brain-derived neurotrophic factor (BDNF). Benefiting from the biocompatibility of GelMA hydrogel, the conductivity of rGO and parallel nanoridge structures of wing scales, PC12 cells, and neural stem cells grown on the modified wing have an increased neurite length with guided cellular orientation. In addition, the NGCs are successfully obtained by manually rolling up the scaffolds and exhibited great performance in repairing 10 mm sciatic nerve defects in rats, and we believe that the NGCs can be applied in reparing longer nerve defects in the future by further optimization. We also demonstrate the feasibility of electrically conductive NGCs based on the rGO/BDNF/GelMA-integrated Morpho butterfly wing as functional nerve regeneration conduits, which may have potential value for application in repairing peripheral nerve injuries.