Male-specific IDGF, a novel gene encoding a membrane-bound extracellular signaling molecule expressed exclusively in testis of Drosophila melanogaster
Male-specific IDGF, a novel gene encoding a membrane-bound extracellular signaling molecule expressed exclusively in testis of Drosophila melanogaster
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DOI：
10.1074/jbc.m003455200
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发表时间：
2000-11-24
影响因子：
4.8
通讯作者：
Natori, S
中科院分区：
文献类型：
--
作者：
Matsushita, T;Fujii-Taira, I;Natori, S
我们发现了一个新的基因，果蝇雄性特异性IDGF（MSI），编码一个跨膜信号分子，只在睾丸中表达。该分子（MSI）含有一个单一的跨膜结构域，并与昆虫衍生的生长因子（IDGF）有35%的氨基酸同一性，IDGF是一种用于肉蝇（Sarcophaga peregrina）胚胎细胞的可溶性生长因子。当MSI在Schneiders 2系细胞中外源表达时，其显示出定位于细胞表面上并表现出生长因子活性，表明MSI是膜结合的细胞外信号分子。基因表达研究表明，MSI mRNA仅限于成熟的初级精母细胞，而MSI检测到的细胞在后期发育阶段。使用四个减数分裂阻滞突变体，aly，can，mia和sa的分析表明，MSI参与精子发生，精子发生的最后分化步骤。这些结果表明MSI是一种参与精子发生的细胞外信号分子，是IDGF家族的一个新成员。
We identified a novel gene of Drosophila melanogaster, Male-specific IDGF (MSI), encoding a transmembrane signaling molecule with exclusive expression in the testis. This molecule (MSI) contains a single transmembrane domain and has 35% amino acid identity with insect-derived growth factor (IDGF), a soluble growth factor for embryonic cells of the flesh fly, Sarcophaga peregrina. When MSI was exogenously expressed in Schneiders's line 2 cells, it was shown to be localized on the cell surface and exhibits growth factor activity, suggesting that MSI is a membrane-bound extracellular signaling molecule. Gene expression studies revealed that MSI mRNA was restricted to mature primary spermatocytes, whereas MSI was detected in the cells at the later developmental stages. Analysis using four meiotic arrest mutants, aly, can, mia, and sa suggested that MSI is involved in spermiogenesis, the final differentiation step of spermatogenesis. These results suggest that MSI is an extracellular signaling molecule participating in spermatogenesis and is a new member of the IDGF family.