Dynamics of lesion processing by bacterial nucleotide excision repair proteins.
Dynamics of lesion processing by bacterial nucleotide excision repair proteins.
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DOI：
10.1016/b978-0-12-387665-2.00001-8
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发表时间：
2012
影响因子：
--
通讯作者：
Van Houten, Bennett
中科院分区：
文献类型：
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作者：
Kad, Neil M.;Van Houten, Bennett
单分子方法允许复杂系统如何运作的无与伦比的观点，最近已被用于了解DNA-蛋白质相互作用。这些工具使得在一个特别具有挑战性的问题上取得了进展，即寻找DNA上的受损位点。DNA修复蛋白在细菌细胞中仅以几百个拷贝的水平存在，在人类细胞中仅以几千个拷贝的水平存在，它们扫描整个基因组以寻找其特定的底物。当这些蛋白质的目标与未受损的DNA可能只存在一个氢键时，它们是如何完成这一艰巨的任务的？在这里，我们研究，使用单分子的方法，原核核苷酸切除修复系统如何平衡的必要性，对特异性的速度。我们在理论，生物和技术层面上讨论问题，最后为未来的研究提出问题。
Single-molecule approaches permit an unrivalled view of how complex systems operate and have recently been used to understand DNA–protein interactions. These tools have enabled advances in a particularly challenging problem, the search for damaged sites on DNA. DNA repair proteins are present at the level of just a few hundred copies in bacterial cells to just a few thousand in human cells, and they scan the entire genome in search of their specific substrates. How do these proteins achieve this herculean task when their targets may differ from undamaged DNA by only a single hydrogen bond? Here we examine, using single-molecule approaches, how the prokaryotic nucleotide excision repair system balances the necessity for speed against specificity. We discuss issues at a theoretical, biological, and technical level and finally pose questions for future research.