Early massive follicle loss and apoptosis in heterotopically grafted newborn mouse ovaries
Early massive follicle loss and apoptosis in heterotopically grafted newborn mouse ovaries
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DOI：
10.1093/humrep/17.3.605
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发表时间：
2002-03-01
影响因子：
6.1
通讯作者：
Dhont, M
中科院分区：
文献类型：
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作者：
Liu, J;Van der Elst, J;Dhont, M
背景：卵巢组织冷冻保存和移植可用于恢复不育女性的生育能力。值得进一步研究的一个问题是卵泡内容物是否受到冻融和移植过程的影响，如果是，影响程度如何以及通过什么机制影响。方法和结果：将完整的新生小鼠卵巢移植到肾被膜下或在移植前用二甲亚砜作为冷冻保护剂缓慢冷冻进行冷冻保存。阴道细胞学检查显示，切除卵巢的受体小鼠的雌激素活性在移植 11 天后恢复。移植后 14 天，回收卵巢移植物并进行组织学处理以进行卵泡数计数。新鲜卵巢移植物的卵泡含量是年龄匹配的14日龄小鼠卵巢卵泡含量的58%。冻融的卵巢移植物中，卵泡含量仅比新鲜移植卵巢低9%。通过 DNA 切口末端标记研究滤泡细胞的凋亡。我们观察到新鲜新生小鼠卵巢移植后不久（2-12 小时）DNA 片段染色显着增加。结论：本研究的结果表明，移植而非冷冻保存是移植新生小鼠卵巢原始卵泡主要和早期丧失的原因。
BACKGROUND: Ovarian tissue cryopreservation and transplantation can be used to restore fertility to sterile females. A question that warrants further investigation is whether the follicular content is affected by the freeze-thawing and grafting procedure, and if so, to what extent and by what mechanism. METHODS AND RESULTS: Intact newborn mouse ovaries were allografted under the kidney capsule or were cryopreserved by slow freezing with dimethylsulphoxide as the cryoprotectant prior to grafting. Estrogenic activity of ovariectomized recipient mice, as revealed by vaginal cytology, resumed after 11 days of transplantation. At 14 days after transplantation, ovarian grafts were recovered and processed histologically for follicle number counting. The follicular content of grafts of fresh ovaries was 58% of that from ovaries of age-matched 14 day old mice. In frozen-thawed ovarian grafts, the follicular content was only 9% lower than that of fresh grafted ovaries. Apoptosis of follicular cells was investigated by DNA nick end labelling. We observed a marked increase in the staining of fragmentation of DNA shortly after transplantation (2-12 h) of fresh newborn mouse ovaries. CONCLUSIONS: The results of the present study indicate that transplantation rather than cryopreservation accounts for the major and early loss of primordial follicles in grafted newborn mouse ovaries.