Characterization of dissolved organic matter in a submerged membrane bioreactor by using three-dimensional excitation and emission matrix fluorescence spectroscopy
Characterization of dissolved organic matter in a submerged membrane bioreactor by using three-dimensional excitation and emission matrix fluorescence spectroscopy
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DOI：
10.1016/j.watres.2008.12.033
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发表时间：
2009-04-01
期刊：
影响因子：
12.8
通讯作者：
Tang, Shujuan
中科院分区：
文献类型：
--
作者：
Wang, Zhiwei;Wu, Zhichao;Tang, Shujuan
采用三维激发发射矩阵 (EEM) 荧光光谱来表征浸没式膜生物反应器 (MBR) 中的溶解有机物 (DOM)。从 MBR 中 DOM 样品的 EEM 荧光光谱中可以识别出三个荧光峰。两个峰与蛋白质样荧光团相关，第三个峰与可见的腐殖酸样荧光团相关。在胞外聚合物（EPS）样品的EEM荧光光谱中仅观察到两个主峰，分别是由于类蛋白质和类腐殖酸物质的荧光。然而，观察到膜污染物的EEM荧光光谱具有三个峰。研究还发现，膜污染物中的主要荧光物质是类蛋白质物质，这可能是由于膜的细孔保留了MBR中DOM和/或EPS中的蛋白质。为了更好地了解DOM、EPS和膜污染物样品的EEM光谱之间的异同，进一步深入了解浸没式MBR中DOM中的荧光物质引起的膜污染，还对荧光光谱（包括峰位置、荧光强度和不同峰强度比）进行了定量分析和荧光区域积分（FRI）分析。 (C) 2008 Elsevier Ltd. 保留所有权利。
Three-dimensional excitation-emission matrix (EEM) fluorescence spectroscopy was employed to characterize dissolved organic matter (DOM) in a submerged membrane bioreactor (MBR). Three fluorescence peaks could be identified from the EEM fluorescence spectra of the DOM samples in the MBR. Two peaks were associated with the protein-like fluorophores, and the third was related to the visible humic acid-like fluorophores. Only two main peaks were observed in the EEM fluorescence spectra of the extracellular polymeric substance (EPS) samples, which were due to the fluorescence of protein-like and humic acid-like matters, respectively. However, the EEM fluorescence spectra of membrane foulants were observed to have three peaks. It was also found that the dominant fluorescence substances in membrane foulants were protein-like substances, which might be due to the retention of proteins in the DOM and/or EPS in the MBR by the fine pores of the membrane. Quantitative analysis of the fluorescence spectra including peak locations, fluorescence intensity, and different peak intensity ratios and the fluorescence regional integration (FRI) analysis were also carried out in order to better understand the similarities and differences among the EEM spectra of the DOM, EPS, and membrane foulant samples and to further provide an insight into membrane fouling caused by the fluorescence substances in the DOM in submerged MBRs. (C) 2008 Elsevier Ltd. All rights reserved.