High resolution-HMBC (HR-HMBC), a new method for measuring heteronuclear long-range coupling constants
High resolution-HMBC (HR-HMBC), a new method for measuring heteronuclear long-range coupling constants
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DOI：
10.1002/mrc.2564
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发表时间：
2010-03-01
影响因子：
2
通讯作者：
Seto, Haruo
中科院分区：
文献类型：
--
作者：
Furihata, Kazuo;Tashiro, Mitsuru;Seto, Haruo
本文提出了一种测量长程C-H偶合常数（J（C-H））的高分辨HMBC（HR-HMBC）脉冲序列。在该脉冲序列中，在自旋演化周期之后结合J缩放脉冲[（nt（1））/2-180度（H/C）-（nt（1））/2]，然后跟随（1）H 180度脉冲以反转J（C-H）耦合的磁化。结果，由于长程J（C-H）引起的交叉峰的分裂与沿F（1）维度的nJ（C-H）的间隔沿着重新对准，因此甚至可以容易地确定小的长程J（C-H）值。在复杂的天然产物波特米星的应用中，已经证明了使用所提出的脉冲序列测量长程J（C-H）的效率。版权所有（c）2010约翰威利父子有限公司。
A useful pulse sequence for measuring long-range C-H coupling constants (J(C-H)) named high resolution-HMBC (HR-HMBC) has been developed. In this pulse sequence, the J-scaling pulse [(nt(1))/2-180 degrees (H/C) -(nt(1))/2] is incorporated after the spin evolution period, and then followed by an (1)H 180 degrees pulse to reverse the magnetization of J(C-H) couplings. As a result, splittings of the cross peaks due to the long-range J(C-H) are realigned with separations of nJ(C-H) along the F(1) dimension, and thus even the small long-range J(C-H) values can easily be determined. The efficiency of measuring the long-range J(C-H) using the proposed pulse sequences has been demonstrated in application to the complicated natural product, portmicin. Copyright (c) 2010 John Wiley & Sons, Ltd.