SPINAL-CORD CONTUSION IN THE RAT - PRODUCTION OF GRADED, REPRODUCIBLE, INJURY GROUPS
SPINAL-CORD CONTUSION IN THE RAT - PRODUCTION OF GRADED, REPRODUCIBLE, INJURY GROUPS
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DOI：
10.1016/0014-4886(85)90117-7
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发表时间：
1985-01-01
影响因子：
5.3
通讯作者：
HARVEY, F
中科院分区：
文献类型：
--
作者：
WRATHALL, JR;PETTEGREW, RK;HARVEY, F
使用重物下落技术在大鼠中产生脊髓挫伤。在T8节段进行限制性椎板切除术，并通过连接至相邻椎骨棘突的夹钳稳定脊柱。将直径为2.4 mm的蓄积器降低到硬脑膜上，并将10 g重量滴在蓄积器上0.0、2.5、5.0、7.5、10.0或17.5 cm处。损伤后4周评估功能缺陷，并对脊髓组织进行组织病理学分析。显然，经受从增加的高度下降的重量的大鼠组（N = 10）表现出分级的最终功能缺陷，如通过改良的Tarlov量表上的分数或Rivlin和Tator的倾斜平面测试中获得的平均角度所测量的。组织学结果也表明了分级病变的产生。实验动物分为轻度、中度和重度三组。这些损伤组中的平均功能缺陷，由重量下降2.5，5.0或17.5 cm产生，在重复实验中是可再现的。这种大鼠脊髓挫伤模型可能有助于筛选假定的治疗药物方案，用于随后对不同脊髓损伤患者组的临床试验。
A weight drop technique was used to produce a contusive injury of the spinal cord in the rat. A restricted laminectomy was carried out at T8 and the spinal column stabilized by clamps attached to the spinous processes of adjacent vertebrae. A 2.4-mm-diameter impounder was lowered onto the dura and a 10-g wt dropped 0.0, 2.5, 5.0, 7.5, 10.0 or 17.5 cm onto the impounder. The functional deficit was assessed for 4 wk after injury and the spinal cord tissue processed for histopathologic analysis. Apparently, groups of rats (N = 10) subjected to the weight dropped from increasing heights exhibited a graded final functional deficit as measured by scores on a modified Tarlov scale or the mean angle attained in the inclinjed plane test of Rivlin and Tator. Histopathologic results also indicated the production of graded lesions. Three groups of experimental animals were statistically distinguished corresponding to those with mild, moderate or severe final functional defict. The average functional deficit in these injury groups, produced by dropping the weight 2.5, 5.0 or 17.5 cm., respectively, was reproducible in replicate experiments. This model of spinal cord contusion in the rat may be useful in screeing putative therapeutic drug regimens for subsequent clinical trials on different groups of patients with spinal cord injury.