INTERFERON ACTION - 2 DISTINCT PATHWAYS FOR INHIBITION OF PROTEIN-SYNTHESIS BY DOUBLE-STRANDED-RNA
INTERFERON ACTION - 2 DISTINCT PATHWAYS FOR INHIBITION OF PROTEIN-SYNTHESIS BY DOUBLE-STRANDED-RNA
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DOI：
10.1073/pnas.75.12.5893
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发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
LENGYEL, P
中科院分区：
文献类型：
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作者：
FARRELL, PJ;SEN, GC;LENGYEL, P
双链RNA至少以两种方式抑制蛋白质合成。它激活一种蛋白激酶，通过磷酸化肽链起始因子 eIF-2 来阻断肽链起始，并且还激活一种核酸内切酶，以不同的速率灭活不同的 mRNA。蛋白激酶和核酸内切酶是从干扰素处理的[小鼠]艾氏腹水肿瘤细胞中部分纯化的。早期发现2'',5''-寡腺苷酸[pppA(2''p5''A)n]是双链RNA激活核酸内切酶的介体，但不是双链RNA激活蛋白激酶的介体。 [这些发现与干扰素的抗病毒作用机制相关]。
Double-stranded RNA inhibits protein synthesis in at least 2 ways. It activates a protein kinase that blocks peptide chain initiation by phosphorylating the peptide chain initiation factor eIF-2 and also activates an endonuclease that inactivates different mRNA at different rates. The protein kinase and the endonuclease were partially purified from interferon-treated [mouse] Ehrlich ascites tumor cells. The 2'',5''-oligoadenylates [pppA(2''p5''A)n], found earlier to be mediators in the activation of the endonuclease by double-stranded RNA, are not mediators in the activation of the protein kinase by double-stranded RNA. [These findings have relevance to the mechanism of the antiviral effect of interferon.].