Non-Immunogenicity of Overlapping Gag Peptides Pulsed on Autologous Cells after Vaccination of HIV Infected Individuals
Non-Immunogenicity of Overlapping Gag Peptides Pulsed on Autologous Cells after Vaccination of HIV Infected Individuals
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DOI：
10.1371/journal.pone.0074389
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发表时间：
2013-10-04
期刊：
影响因子：
3.7
通讯作者：
Goulder, Philip
中科院分区：
文献类型：
--
作者：
Kloverpris, Henrik N.;Jackson, Akil;Goulder, Philip
背景：HIV Gag特异性CD 4+和CD 8 + T细胞应答对于HIV免疫控制非常重要。脉冲重叠Gag肽自体淋巴细胞（OPAL）已被证明免疫原性和有效地减少病毒载量在多个猪尾猕猴studies，cancerting clinical evaluation.Methodology：我们进行了I期，单中心，安慰剂对照，双盲和剂量递增的研究，以评估安全性和初步免疫原性的一种新的治疗性疫苗的方法'OPAL-HIV-Gag（C）'。该疫苗由120个15聚体肽组成，重叠11个氨基酸，跨越HIV Gag C进化枝序列蛋白质组，使用封闭系统在从全血富集的白色血细胞上脉冲，然后静脉内再输注。HIV病毒载量检测不到的患者（
Background: HIV Gag-specific CD4+ and CD8+ T-cell responses are important for HIV immune control. Pulsing overlapping Gag peptides on autologous lymphocytes (OPAL) has proven immunogenic and effective in reducing viral loads in multiple pigtail macaque studies, warranting clinical evaluation.Methodology: We performed a phase I, single centre, placebo-controlled, double-blinded and dose-escalating study to evaluate the safety and preliminary immunogenicity of a novel therapeutic vaccine approach 'OPAL-HIV-Gag(c)'. This vaccine is comprised of 120 15mer peptides, overlapping by 11 amino acids, spanning the HIV Gag C clade sequence proteome, pulsed on white blood cells enriched from whole blood using a closed system, followed by intravenous reinfusion. Patients with undetectable HIV viral loads (