Improved gateway binary vectors: High-performance vectors for creation of fusion constructs in Transgenic analysis of plants
Improved gateway binary vectors: High-performance vectors for creation of fusion constructs in Transgenic analysis of plants
复制标题
DOI：
10.1271/bbb.70216
复制
发表时间：
2007-08-01
影响因子：
1.6
通讯作者：
Ishiguro, Sumie
中科院分区：
文献类型：
--
作者：
Nakagawa, Tsuyoshi;Suzuki, Takarnasa;Ishiguro, Sumie
我们构建了一系列用于植物转化的改进的Gateway二元载体(PGWB)。使用了15种不同的记者和标签，sGFP、GUS、Luc、EYFP、ECFP、G3GFP、MRFP、6xHis、FLAG、3xHA、4xMyc、10xMyc、GST、T7和TAP。有些载体携带2x35S-Omega启动子以实现更高水平的表达。43种载体中的每一种都独立提供了卡那霉素和潮霉素抗性标记，因此可以很容易地对一次转化的植物进行额外的转化。它们在大肠杆菌中的小尺寸和高拷贝数使质粒制备和测序变得更容易处理。改良的pGWBS有望成为植物转基因研究的有力工具。
We made a series of improved Gateway binary vectors (pGWBs) for plant transformation. Fifteen different reporters and tags, sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7, and TAP, were employed. Some vectors carry the 2x35S-Omega promoter for higher-level expression. The kanamycin- and hygromycin-resistant markers are independently available for each of the 43 types of vectors, thus an additional transformation of once- transformed plants can be carried out easily. Their small size and high-copy number in Escherichia coli make possible easier handling at plasmid preparation and sequencing. Improved pGWBs should be a powerful tool for transgenic research in plants.