G protein βγ11 complex translocation is induced by Gi, Gq and Gs coupling receptors and is regulated by the α subunit type
G protein βγ11 complex translocation is induced by Gi, Gq and Gs coupling receptors and is regulated by the α subunit type
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DOI：
10.1016/j.cellsig.2005.09.007
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发表时间：
2006-08-01
影响因子：
4.8
通讯作者：
Gautam, N.
中科院分区：
文献类型：
--
作者：
Azpiazu, Inaki;Akgoz, Muslum;Gautam, N.
通过Gi/Go偶联M2毒蕈碱受体、Gq偶联M3受体和Gs偶联2肾上腺素能受体的G蛋白活化引起G蛋白γ 11亚基从质膜到高尔基复合体的快速可逆易位。I亚基的共易位表明γ 11易位为β-γ复合物。百日咳毒素ADP核糖基化α i亚基类型或α o的C末端结构域被α s的相应区域取代抑制γ 11易位，表明亚基与受体的相互作用及其活化是易位的必要条件。γ 11易位的速率对G蛋白a亚基的活化速率敏感。与具有相对较低鸟嘌呤核苷酸交换速率的α亚基类型相比，在体外显示高受体活化鸟嘌呤核苷酸交换速率的α亚基类型支持高γ 11易位速率。结果表明，受体诱导的γ 11易位是由G蛋白通过活性和非活性形式的循环速率控制的。他们还证明，γ 11易位的成像可用作非侵入性工具来测量活细胞中野生型或突变型受体和α亚基类型的相对活性。(c)2005年爱思唯尔公司All rights reserved.
G protein activation by Gi/Go coupling M2 muscarinic receptors, Gq coupling M3 receptors and Gs coupling 2 adrenergic receptors causes rapid reversible translocation of the G protein gamma 11 subunit from the plasma membrane to the Golgi complex. Co-translocation of the I subunit suggests that gamma 11 translocates as a beta gamma complex. Pertussis toxin ADP ribosylation of the alpha i subunit type or substitution of the C terminal domain of alpha o with the corresponding region of alpha s inhibits gamma 11 translocation demonstrating that a subunit interaction with a receptor and its activation are requirements for the translocation. The rate of gamma 11 translocation is sensitive to the rate of activation of the G protein a subunit. (x subunit types that show high receptor activated rates of guanine nucleotide exchange in vitro support high rates of gamma 11 translocation compared to a subunit types that have a relatively lower rate of guanine nucleotide exchange. The results suggest that the receptor induced translocation of gamma 11 is controlled by the rate of cycling of the G protein through active and inactive forms. They also demonstrate that imaging of gamma 11 translocation can be used as a non-invasive tool to measure the relative activities of wild type or mutant receptor and alpha subunit types in a live cell. (c) 2005 Elsevier Inc. All rights reserved.