Rescue of shoot apical meristems of chrysanthemum by culturing on root tips
Rescue of shoot apical meristems of chrysanthemum by culturing on root tips
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DOI：
10.1007/s00299-003-0719-1
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发表时间：
2004-02-01
影响因子：
6.2
通讯作者：
Yazawa, S
中科院分区：
文献类型：
--
作者：
Hosokawa, M;Otake, A;Yazawa, S
建立了一种新的无叶原基茎尖分生组织圆顶（LP-free SAMs）再生植株的方法。培养10 d后，贴附于菊花根尖的“Piato”无脂自组装膜的存活率> 40%。芽再生没有观察到从LP-自由SAM没有根尖。当将菊花的无脂自组装膜转移到矮牵牛、甘蓝或康乃馨的根尖时，观察到甘蓝根尖的芽再生率最高。显微镜观察证明，无LP的SAM暂时粘附在甘蓝根尖的切割表面上。
A new method to regenerate plants from leaf primordia-free shoot apical meristem domes (LP-free SAMs) was developed by establishing the meristem dome on the cut surface of root tips. Ten days after culture, the viable rate of LP-free SAMs of chrysanthemum 'Piato' attached to chrysanthemum root tips was >40%. Shoot regeneration was not observed from LP-free SAMs without the root tips. When LP-free SAMs of chrysanthemum were transferred to root tips of either petunia, cabbage, or carnation, the highest shoot regeneration rate was observed with cabbage root tips. Microscopic observation documented that the LP-free SAM temporarily adhered to the cut surface of the root tip of cabbage.