An aphid effector promotes barley susceptibility through suppression of defence gene expression
An aphid effector promotes barley susceptibility through suppression of defence gene expression
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DOI：
10.1101/639476
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发表时间：
2019-05
期刊：
影响因子：
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通讯作者：
Carmen M. Escudero-Martinez;Patricia A. Rodriguez;Pablo A. Santos;J. Stephens;J. Bos
中科院分区：
文献类型：
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作者：
Carmen M. Escudero-Martinez;Patricia A. Rodriguez;Pablo A. Santos;J. Stephens;J. Bos
蚜虫分泌不同的效应子库到它们的宿主中以促进侵染过程。虽然“组学”方法有助于鉴定和比较来自许多蚜虫物种的效应子库，但这些蛋白质的功能表征仅限于双子叶植物（模式）。禾谷缢管蚜（Rhopalosiphum padi）是禾谷类作物（包括大麦）的害虫。在这里，我们扩大了努力，以表征蚜虫效应器方面的作用，促进易感性的R。padi-barley相互作用我们选择了3R。padi效应子基于与先前表征的M.桃效应子，并评估其亚细胞定位，表达和促进植物易感性的作用。表达R.转基因大麦中的padi效应子RpC 002和Rp 1增强了植株对赤霉病的敏感性。padi而不是M.桃蚜，大麦是一个贫穷的主机。Rp 1转基因大麦株系的特征显示，植物激素信号转导基因的基因表达减少，植物蚜虫的相互作用，表明这种效应增强易感性，抑制植物防御大麦。我们的数据表明，一些蚜虫效应器在宿主物种中表达时特异性地起作用，并且具有有益于其相应蚜虫物种的活动。
Aphids secrete diverse repertoires of effectors into their hosts to promote the infestation process. While “omics”-approaches facilitated the identification and comparison of effector repertoires from a number of aphid species, the functional characterization of these proteins has been limited to dicot (model) plants. The bird cherry-oat aphid Rhopalosiphum padi is a pest of cereal crops, including barley. Here, we extended efforts to characterize aphid effectors with regards to their role in promoting susceptibility to the R. padi-barley interaction. We selected 3 R. padi effectors based on sequences similarity to previously characterized M. persicae effectors and assessed their subcellular localisation, expression, and role in promoting plant susceptibility. Expression of R. padi effectors RpC002 and Rp1 in transgenic barley lines enhanced plant susceptibility to R. padi but not M. persicae, for which barley is a poor host. Characterization of Rp1 transgenic barley lines revealed reduced gene expression of plant hormone signalling genes relevant to plant-aphid interactions, indicating this effector enhances susceptibility by suppressing plant defences in barley. Our data suggests that some aphid effectors specifically function when expressed in host species, and feature activities that benefit their corresponding aphid species.