Detection of dairy Leuconostoc strains using the polymerase chain reaction
Detection of dairy Leuconostoc strains using the polymerase chain reaction
复制标题
使用聚合酶链式反应检测乳制品明串珠菌菌株
DOI：
10.1111/j.1472-765x.1995.tb00428.x
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发表时间：
1995
影响因子：
2.4
通讯作者：
G. Davey
中科院分区：
文献类型：
--
作者：
L. Ward;J.C. Brown;G. Davey
本文报道了基于16S rRNA序列数据的明串珠菌特异性寡核苷酸的设计。当将该寡核苷酸与16S rRNA序列保守区的寡核苷酸一起用于聚合酶链式反应(PCR)时，产生了约470个碱基的明串珠菌特异的PCR产物。在保守区域使用第二个寡核苷酸，可以在所有PCR中产生大约350bp的产物，作为阳性对照。所述的聚合酶链式反应程序特别适用于检测混合中温发酵剂培养物中是否存在明串珠菌。明珠藻特异的寡核苷酸也被用作特异的杂交探针。
This paper reports the design of a Leuconostoc‐specific oligonucleotide based on 16S rRNA sequence data. When this oligonucleotide was used in a polymerase chain reaction (PCR) in conjunction with an oligonucleotide to a conserved region of the 16S rRNA sequence, a Leuconostoc‐specific PCR product of approximately 470 bp was produced. The use of a second oligonucleotide to a conserved region allowed the production of an approximately 350 bp product in all PCRs, acting as a positive control. The PCR procedure described was particularly useful for detecting the presence of Leuconostoc in mixed mesophilic starter cultures. The Leuconostoc‐specific oligonucleotide was used also as a specific hybridization probe.