Evaluation of Lasting Effects of Heat Stress on Sperm Profile and Oxidative Status of Ram Semen and Epididymal Sperm.
Evaluation of Lasting Effects of Heat Stress on Sperm Profile and Oxidative Status of Ram Semen and Epididymal Sperm.
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DOI：
10.1155/2016/1687657
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发表时间：
2016
影响因子：
--
通讯作者：
Assumpção ME
中科院分区：
文献类型：
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作者：
Hamilton TR;Mendes CM;de Castro LS;de Assis PM;Siqueira AF;Delgado Jde C;Goissis MD;Muiño-Blanco T;Cebrián-Pérez JÁ;Nichi M;Visintin JA;Assumpção ME
较高的温度会导致睾丸新陈代谢增加，从而导致精子损伤。氧化应激是热应激导致睾丸损伤的主要因素。本研究的目的是评估热应激对射出精子的持久影响以及热应激对附睾精的即刻或长期影响。我们观察到治疗组射出精子的活动率和质量活动率下降，出现严重和轻微缺陷的精子百分率增加，顶体膜和质膜受损的精子百分率增加，高线粒体膜电位的精子百分率下降直到一个精子周期。治疗组射精液中谷胱甘肽过氧化物酶活性升高，应激细胞增多。治疗组小鼠精子线粒体膜电位升高的百分率下降。然而，当比较即刻和长期影响时，我们观察到低线粒体膜电位的精子百分比增加。总而言之，睾丸热应激诱导氧化应激，导致射精精子在一个精子周期后发生可挽救的变化，在附睾精中也在30天后发生改变。
Higher temperatures lead to an increase of testicular metabolism that results in spermatic damage. Oxidative stress is the main factor responsible for testicular damage caused by heat stress. The aim of this study was to evaluate lasting effects of heat stress on ejaculated sperm and immediate or long-term effects of heat stress on epididymal sperm. We observed decrease in motility and mass motility of ejaculated sperm, as well as an increase in the percentages of sperm showing major and minor defects, damaged plasma and acrosome membranes, and a decrease in the percentage of sperm with high mitochondrial membrane potential in the treated group until one spermatic cycle. An increased enzymatic activity of glutathione peroxidase and an increase of stressed cells were observed in ejaculated sperm of the treated group. A decrease in the percentage of epididymal sperm with high mitochondrial membrane potential was observed in the treated group. However, when comparing immediate and long-term effects, we observed an increase in the percentage of sperm with low mitochondrial membrane potential. In conclusion, testicular heat stress induced oxidative stress that led to rescuable alterations after one spermatic cycle in ejaculated sperm and also after 30 days in epididymal sperm.