Elevation of carbohydrate antigen 125 in chronic heart failure may be caused by mechanical extension of mesothelial cells from serous cavity effusion
Elevation of carbohydrate antigen 125 in chronic heart failure may be caused by mechanical extension of mesothelial cells from serous cavity effusion
复制标题
慢性心力衰竭中碳水化合物抗原125的升高可能是由浆膜腔积液中的间皮细胞的机械延伸引起的。
DOI：
10.1016/j.clinbiochem.2013.09.008
复制
发表时间：
2013-11-01
影响因子：
2.8
通讯作者：
Huang, Hui
中科院分区：
文献类型：
--
作者：
Huang, Feifei;Zhang, Kun;Huang, Hui
目的：升高的碳水化合物抗原125（CA 125）预测慢性心力衰竭（CHF）的临床结果的实际应用正在争论中。CM 25升高的机制尚不清楚。我们假设，对间皮细胞的机械应力启动CA 125 synthesis.Design和方法：共191例患有水肿和/或呼吸困难的患者参加。CHF组109例，对照组82例。检测超声心动图、CA 125、N末端脑钠肽前体（NT-proBNP）和其他生化参数。结果：有浆膜腔积液（SCE）者血清CA 125水平明显高于无SCE者（82.91（61.90-103.92）vs.44.98（29.66-60.30）U/mL，P < 0.001）。在无SCE的情况下，CHF患者的CA 125水平略高于非CHF患者（52.37（34.85-69.90）vs. 35.15（23.81-46.49）U/mL，P = 0.017）。此外，与非CHF患者相比，CHF患者的高敏C反应蛋白（hsCRP）水平较高，超氧化物歧化酶（SOD）水平较低。所有入选患者血清CA 125水平与SOD水平呈负相关（r =-0.567，P < 0.001），与hsCRP水平呈正相关（r = 0.608，P < 0.001）。受试者工作特征曲线分析显示，CA 125对SCE的预测优于NT-proBNP，而NT-proBNP对CHF的预测优于CA 125。体外研究表明，机械牵张可上调人间皮细胞系（MeT-5A）CA 125编码基因MUC 16的表达。间皮细胞从SCE的机械延伸在CA 125增加中起重要作用。(C)2013年加拿大临床化学家协会。爱思唯尔公司出版All rights reserved.
Objectives: The practical application of elevated carbohydrate antigen 125 (CA125) to predict clinical outcome in chronic heart failure (CHF) is under debate. The mechanism for this CM 25 elevation remains unknown. We hypothesize that mechanical stress on mesothelial cells initiates CA125 synthesis.Design and methods: A total of 191 patients suffering from edema and/or dyspnea were enrolled. 109 patients were diagnosed as CHF, and 82 patients without CHF were assigned as control group. Echocardiography, CA125, N-terminal pro-brain natriuretic peptide (NT-proBNP), and other biochemical parameters were measured. All enrolled patients underwent heart function classification.Results: Patients with serous cavity effusion (SCE) demonstrated higher serum CA125 than patients without SCE (82.91 (61.90-103.92) vs. 44.98 (29.66-60.30) U/mL, P < 0.001). In the absence of SCE, CA125 levels in CHF patients were slightly higher than non-CHF patients (52.37 (34.85-69.90) vs. 35.15 (23.81-46.49) U/mL, P = 0.017). Additionally, compared with non-CHF patients, CHF patients had higher levels of high-sensitivity C-reactive protein (hsCRP) and lower superoxide dismutase (SOD). In all enrolled patients, CA125 levels were negatively correlated with SOD concentrations (r = -0.567, P < 0.001), and positively correlated with hsCRP levels (r = 0.608, P < 0.001). Receiver operating characteristic curve analysis showed that CA125 was better in predicting SCE than NT-proBNP, while NT-proBNP was more suitable for predicting CHF than CA125. The in vitro study demonstrated that MUC16, the CA125 coding gene, was up-regulated by mechanical stretch on human mesothelial cell line (MeT-5A).Conclusions: CA125 elevation in CHF was associated with SCE. Mechanical extension of mesothelial cells from SCE plays an important role in CA125 increase. (C) 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.