GSPE is superior to NAC in the prevention of contrast-induced nephropathy: Might this superiority be related to caspase 1 and calpain 1?
GSPE is superior to NAC in the prevention of contrast-induced nephropathy: Might this superiority be related to caspase 1 and calpain 1?
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DOI：
10.1016/j.lfs.2014.03.030
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发表时间：
2014-05-17
期刊：
影响因子：
6.1
通讯作者：
Yucesan, Fulya Balaban
中科院分区：
文献类型：
--
作者：
Ulusoy, Sukru;Ozkan, Gulsum;Yucesan, Fulya Balaban
目的：本研究旨在探讨诱导型一氧化氮合酶（inducible nitric oxide synthase，iNOS）、内皮型一氧化氮合酶（endothelial nitric oxide synthase，eNOS）、半胱氨酸蛋白酶1（caspase 1）、半胱氨酸蛋白酶3（caspase 3）和钙蛋白酶1（calpain 1）在造影剂肾病（contrast induced nephropathy，CIN）发病中的作用，并比较N乙酰半胱氨酸（NAC）和葡萄籽原花青素提取物（GSPE）对CIN的保护作用。对照、造影剂（CM）、CM + NAC和CM + GSPE。用7 ml/kg泛影葡胺诱发CIN。实验在第九天停止。采集血液用于血尿素氮（BUN）和肌酐测量。结果：与对照组相比，CM组大鼠BUN、肌酐、肾组织病理学损伤、TUNEL、caspase 1、3、calpain 1、iNOS和eNOS均显著增加。CM + GSPE组上述指标均有改善，而CM + NAC组BUN、肌酐及肾组织病理学损伤无明显改善。此外，钙蛋白酶1染色和肌酐显着降低CM + GSPE组相比，CM + NACgroup.Significance：我们的研究表明，在文献中首次，GSPE具有更大的肾保护作用相比，NAC，这种有效的保护可能与钙蛋白酶1水平降低。(C)2014爱思唯尔公司All rights reserved.
Aims: Our study was intended to evaluate the role of inducible nitric oxide synthase (iNOS), endothelial nitric oxide synthase (eNOS), caspases 1 and 3 and calpain 1 in the pathogenesis of contrast-induced nephropathy (CIN) and to compare the protective effects of N acetyl cysteine (NAC) and grape seed proanthocyanidin extract (GSPE) against the development of CIN.Main methods: 32 rats were divided into four groups; control, contrast media (CM), CM + NAC and CM + GSPE. CIN was induced by administration of 7 ml/kg diatrizoate. The experiment was discontinued on the ninth day. Blood was collected for blood urea nitrogen (BUN) and creatinine measurement. Rat kidney tissues were removed for histopathological evaluation and the investigation of caspases 1 and 3, iNOS, eNOS, TUNEL and calpain 1.Key findings: A significant increase in BUN, creatinine, renal histopathological injury, TUNEL, caspases 1,3, calpain 1, iNOS and eNOS was observed in the CM group compared to the control group. There was amelioration in all these parameters in the CM + GSPE group, while there was no significant amelioration in BUN, creatinine and renal histopathological injury in the CM + NAC group. In addition, calpain 1 staining and creatinine were significantly lower in the CM + GSPE group compared to the CM + NAC group.Significance: Our study showed, for the first time in the literature, that GSPE has a greater renoprotective effect compared with NAC and that this effective protection may be related to decrease in calpain 1 levels. (C) 2014 Elsevier Inc. All rights reserved.