Statistical Validation Verifies That Enantiomorphic States of Chiral Cells Are Determinant Dictating the Left- or Right-Handed Direction of the Hindgut Rotation in Drosophila
Statistical Validation Verifies That Enantiomorphic States of Chiral Cells Are Determinant Dictating the Left- or Right-Handed Direction of the Hindgut Rotation in Drosophila
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DOI：
10.3390/sym12121991
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发表时间：
2020-12
期刊：
影响因子：
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通讯作者：
Tomoki Ishibashi;Mikiko Inaki;K. Matsuno
中科院分区：
文献类型：
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作者：
Tomoki Ishibashi;Mikiko Inaki;K. Matsuno
在无脊椎动物的左右（LR）不对称发育中，细胞手性至关重要。左手或右手细胞结构通过相应的 LR 不对称性指导形态发生。在果蝇中，细胞手性被认为驱动胚胎后肠和其他器官的 LR 不对称发育。这一假设仅得到细胞手性的 LR 方向性和后肠旋转之间明显的一致性以及连接这两个事件的计算机模拟的支持。在本文中，我们从数学角度评估了后肠上皮细胞的手性与后肠旋转的 LR 方向之间的因果关系。我们从几种果蝇基因型中得出的逻辑模型，显着解释了手性细胞的对映体（左旋或右旋）状态与后肠旋转的LR方向性之间的相关性——即使在具有随机确定的细胞手性和随机后肠旋转的个体活突变体胚胎中，这表明细胞手性形成的机制与后肠旋转的方向无关。因此，我们的分析表明，在后肠旋转之前形成的细胞手性对于随后的旋转来说既是充分的也是必需的，验证了细胞手性因果地定义后肠旋转的LR方向性的假设。
In the left–right (LR) asymmetric development of invertebrates, cell chirality is crucial. A left- or right-handed cell structure directs morphogenesis with corresponding LR-asymmetry. In Drosophila, cell chirality is thought to drive the LR-asymmetric development of the embryonic hindgut and other organs. This hypothesis is supported only by an apparent concordance between the LR-directionality of cell chirality and hindgut rotation and by computer simulations that connect the two events. In this article, we mathematically evaluated the causal relationship between the chirality of the hindgut epithelial cells and the LR-direction of hindgut rotation. Our logistic model, drawn from several Drosophila genotypes, significantly explained the correlation between the enantiomorphic (sinistral or dextral) state of chiral cells and the LR-directionality of hindgut rotation—even in individual live mutant embryos with stochastically determined cell chirality and randomized hindgut rotation, suggesting that the mechanism by which cell chirality forms is irrelevant to the direction of hindgut rotation. Thus, our analysis showed that cell chirality, which forms before hindgut rotation, is both sufficient and required for the subsequent rotation, validating the hypothesis that cell chirality causally defines the LR-directionality of hindgut rotation.