Antibacterial activity of Lentinula edodes grown in liquid medium
Antibacterial activity of Lentinula edodes grown in liquid medium
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DOI：
10.1590/s1517-83822001000300008
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发表时间：
2001-07-01
影响因子：
2.2
通讯作者：
Vanetti, MCD
中科院分区：
文献类型：
--
作者：
Ishikawa, NK;Kasuya, MCM;Vanetti, MCD
采用半固体覆盖琼脂扩散技术，评价了35个香菇分离物对枯草杆菌的抗菌活性。所有菌株均抑制B。枯草芽孢杆菌和分离物Le 1促进了最大抑菌圈的形成。L. edodes Le 1对食源性致病菌和食品污染菌也具有抗菌活性，特别是革兰氏阳性菌。培养18-25天的培养滤液的抑菌活性。在25 ℃的发酵液中，香菇的产量较高。当滤液在乙酸乙酯和水之间分配时，仅在有机层中观察到抑制活性，表明抑制物质具有低极性。在氯仿-丙酮-乙酸乙酯-甲醇（40：5：5：2）中形成的硅胶薄层区（Rf值为0.63-0.80）是对B具有抗菌活性的区域。枯草杆菌。L.在100 ℃热处理10分钟和4 ℃保存120天后，在培养滤液中可检测到菌丝体。
The antibacterial activity of 35 isolates of Lentinula edodes, a shiitake mushroom, against Bacillus subtilis was evaluated by diffusion technique in agar with a semi-solid overlay. All isolates inhibited B. subtilis and the isolate Le1 promoted the formation of the largest inhibition zone. L. edodes Le1 also presented antibacterial activity against foodborne pathogens and food contaminant bacteria, particularly Gram-positive species. The antibacterial activity of the culture filtrate after 18-25 days of cultivation of L. edodes in broth at 25degreesC was high. The inhibitory activity was observed only in the organic layer when the culture filtrate was partitioned between ethyl acetate and water, suggesting that the inhibitory substances have low polarity. The silica gel thin-layer zone at Rf values of 0.63-0.80, developed in chloroform - acetone ethyl acetate - methanol 40:5:5:2, was responsible for the antibacterial activity against B. subtilis. The inhibitory activity of L. edodes was detectable in the culture filtrate after heat treatment at 100degreesC for 10 min and after storage at 4degreesC for 120 days.