2-DIMENSIONAL IMAGING OF O-2, H2O2, AND GLUCOSE DISTRIBUTIONS BY AN ARRAY OF 400 INDIVIDUALLY ADDRESSABLE MICROELECTRODES
2-DIMENSIONAL IMAGING OF O-2, H2O2, AND GLUCOSE DISTRIBUTIONS BY AN ARRAY OF 400 INDIVIDUALLY ADDRESSABLE MICROELECTRODES
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DOI：
10.1021/ac00103a005
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发表时间：
1995-04-01
影响因子：
7.4
通讯作者：
ROSPERT, M
中科院分区：
文献类型：
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作者：
MEYER, H;DREWER, H;ROSPERT, M
采用改进的 CMOS 工艺制造了 400 个可单独寻址的微电极的单片阵列。每个铂电极尺寸为36μm x 36μm，排列成总面积为1cm(2)的方阵。阵列中每个电极的单独寻址是通过两个片上移位寄存器与阵列控制逻辑耦合来实现的。在电化学扫描模式下，连续读取400个电极信号。该阵列用于支持电解质溶液，以在 -0.8 V vs Ag/AgCl 条件下对氧分布进行电流分析检测。过氧化氢和葡萄糖分布在 +0.8 V vs Ag/AgCl 下通过电流分析成像。在对葡萄糖分布进行成像之前，葡萄糖氧化酶同时被捕获到所有阵列电极上的导电聚合物聚吡咯中。连续读出所有 400 个微电极信号需要两分钟。通过一系列连续扫描以时间分辨方式对分析物的扩散进行成像。因此，可单独寻址的微电极阵列可以被视为扫描电化学显微镜的替代电化学成像装置。
A monolithic array of 400 individually addressable microelectrodes was fabricated in a modified CMOS process. The platinum electrodes, each 36 mu m x 36 mu m in size, were arranged in a square matrix of overall area 1 cm(2). Individual addressing of each electrode in the array was realized by two on-chip shift registers coupled with array control logic. In the electrochemical scanning mode, the 400 electrode signals were read successively. The array was used in supporting electrolyte solutions for imaging of oxygen distributions detected amperometrically at -0.8 V vs Ag/AgCl. Hydrogen peroxide and glucose distributions were imaged amperometrically at +0.8 V vs Ag/AgCl. Prior to imaging glucose distributions, the enzyme glucose oxidase was entrapped into the conducting polymer polypyrrole simultaneously on all array electrodes. Two minutes was needed for a successive readout of all 400 microelectrode signals. Diffusion of the analytes was imaged in a time-resolved manner by a sequence of successive scans. Therefore, the array of individually addressable microelectrodes can be regarded as an alternative electrochemical imaging device to the scanning electrochemical microscope.