Microelectronic cell sensor assay for detection of cytotoxicity and prediction of acute toxicity
Microelectronic cell sensor assay for detection of cytotoxicity and prediction of acute toxicity
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DOI：
10.1016/j.tiv.2005.12.008
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发表时间：
2006-09-01
影响因子：
3.2
通讯作者：
Xie, Li
中科院分区：
文献类型：
--
作者：
Xing, James Z.;Zhu, Lijun;Xie, Li
本研究报告了实时细胞电子传感（RT-CES）系统的内部评估，该系统用作细胞毒性试验和预测急性毒性的试验平台。对于细胞毒性测定，RT-CES试验显示出与NRU试验相同的灵敏度和变异系数值，具有良好的相关性。在拟定的啮齿类动物急性毒性预测模型背景下，比较细胞毒性参比物质的IC 50值和LD 50值。从RT-CES试验获得的结果符合预测模型的验收限度，并表明RT-CES细胞毒性试验符合NIH出版物#01-4500中准确预测急性毒性的确认指南。除细胞活力外，RT-CES试验还提供了动态信息，可用于鉴定终点试验难以达到的最大毒性和毒性作用的可逆性，因此，RT-CES试验更准确地评估细胞毒性。RT-CES法具有免标记、自动检测、操作简便等特点，有望取代BALB/c3 T3 NRU法，作为毒理学实验室药物监测的常规方法。(c)2006爱思唯尔有限公司保留所有权利。
This study reports in-house assessment of a real-time cell electronic sensing (RT-CES) system used as a test platform for both cytotoxicity assay and predicting acute toxicity. For cytotoxicity determination, the RT-CES assay displayed equal sensitivity and coefficients of variation values with good correlation to NRU assay. The IC50 values and the LD50 values for the cytotoxicity reference materials were compared in the context of the proposed prediction model for acute rodent toxicity. The results obtained from RT-CES assay fitted within the acceptance limits of the prediction model and showed that the RT-CES cytotoxicity assay met the qualification guidelines in NIH Publication #01-4500 to accurately predict acute toxicity. In addition to cell viability, the RT-CES assay provided dynamic information that can be used to identify maximum toxicity and reversibility of the toxic effects which are difficult to achieve by the endpoint assays and, therefore, the RT-CES assay is more accurate for assessment of cytotoxicity. The features of the RT-CES assay, such as labeling free, automatic detection, and easy operation, give this assay potential to replace BALB/c3T3 NRU assay and be used as routine setting for drug monitoring in the toxicological laboratory. (c) 2006 Elsevier Ltd. All rights reserved.