Evaluation of a Suitable DNA Damage Biomarker for Human Biomonitoring of Exposed Workers
Evaluation of a Suitable DNA Damage Biomarker for Human Biomonitoring of Exposed Workers
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DOI：
10.1002/em.20501
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发表时间：
2009-12-01
影响因子：
2.8
通讯作者：
Iavicoli, Sergio
中科院分区：
文献类型：
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作者：
Cavallo, Delia;Ursini, Cinzia Lucia;Iavicoli, Sergio
本研究的目的是确定一种敏感且无创的早期遗传毒性生物标志物，用于暴露于低剂量外源药物混合物的工人的健康风险评估。我们研究了30名接触抗肿瘤药物的工人，57名接触不同混合物多环芳烃（PAHs）的工人（41名机场工人和16名铺路工人）和76名对照者。对淋巴细胞和脱落的颊细胞进行彗星和微核（MN）试验。淋巴细胞的MN测定在暴露者和对照组之间没有显着差异，而对脱落的颊细胞的MN测定在暴露于抗肿瘤药物的工人中显示高于对照组（0.85比0.48,P = 0.042）。多环芳烃暴露个体的淋巴细胞彗星检测显示，与对照组相比，多环芳烃暴露个体的彗星百分比值（18.11比11.24,P = 0.001）和平均尾矩（TM）值（21.84比16.72,P = 0.003）高于对照组；暴露于抗肿瘤剂中没有发现显著差异。对脱落的口腔细胞进行彗星测定，暴露组和对照组之间的彗星百分比没有显着差异，而暴露于多环芳烃的工人的TM值更高（55.1比32.31,P < 0.001）。这些结果表明，通过非侵入性方法获得的脱落的口腔细胞是评估低剂量可吸入化学物质混合物职业性暴露的可靠靶细胞。彗星试验似乎适合于迅速评估含有挥发性物质的多环芳烃混合物的遗传毒性作用。MN试验适用于评价抗肿瘤药物的效果。环绕。生物工程学报，2009。(C) 2009 Wiley-Liss, Inc。
The objective of this study was to identify a sensitive and noninvasive biomarker of early genotoxic effects, for health risk assessment of workers exposed to mixtures of low doses of xenobiotics. We studied 30 workers exposed to antineoplastic drugs, 57 workers exposed to different mixtures of polycyclic aromatic hydrocarbons (PAHs) (41 airport workers and 16 paving workers) and 76 controls. Comet and micronucleus (MN) tests were performed on lymphocytes and exfoliated buccal cells. The MN assay on lymphocytes did not show significant differences between exposed and controls, while the MN assay on exfoliated buccal cells showed higher values in workers exposed to antineoplastics as compared with controls (0.85 vs. 0.48, P = 0.042). The comet assay on lymphocytes showed a higher comet percentage value (18.11 vs. 11.24 in controls, P = 0.001) and mean tail moment (TM) value (21.84 vs. 16.72 in controls, P = 0.003) in individuals exposed to PAHs as compared with controls; no significant differences were found in exposed to antineoplastics. The comet assay on exfoliated buccal cells did not show significant differences between exposed and control groups for comet percentages, whereas the TM value was higher in workers exposed to PAHs (55.1 vs. 32.31 for controls, P < 0.001). These results show that exfoliated buccal cells, obtained by a noninvasive procedure, represent robust target cells to assess the occupational exposure to inhalable mixture of chemicals at low doses. The comet assay seems to be suitable to promptly evaluate the genotoxic effects of PAHs mixtures that also contain volatile substances. The MN test is suitable to evaluate the effects of antineoplostics. Environ. Mal. Mutagen. 50:781-790, 2009. (C) 2009 Wiley-Liss, Inc.