Human olfactory receptor neurons contain OMP mRNA in their dendritic and axonal processes.
Human olfactory receptor neurons contain OMP mRNA in their dendritic and axonal processes.
复制标题
人类嗅觉受体神经元的树突和轴突中含有 OMP mRNA。
DOI：
10.1097/00001756-199503270-00028
复制
发表时间：
1995
期刊：
影响因子：
1.7
通讯作者：
Getchell,TV
中科院分区：
文献类型：
--
作者：
Krishna,NS;Getchell,ML;Buiakova,OI;Margolis,FL;Getchell,TV
采用原位杂交和免疫细胞化学方法，研究了26周龄至85岁人嗅粘膜中嗅觉标记蛋白（OMP）mRNA和蛋白的细胞表达。OMP mRNA和蛋白在嗅感受器神经元胞体中含量最高。ORN体层的杂交信号在老年受试者中比在年轻受试者中更大，反映了更高的神经元密度或每个细胞更多的OMP mRNA。相比之下，与年龄匹配的对照组相比，阿尔茨海默病受试者的这一比例明显较低。老年受试者的特点是斑块分布的OMP表达的ORN和发生上皮下内陷含有OMP阳性神经元。此外，一个显着的杂交信号中检测到的顶端嗅上皮含有树突，树突状旋钮，纤毛的ORNS，并在嗅神经束的固有层，表明OMP mRNA的发生在树突和轴突域。
THE cellular expression of olfactory marker protein (OMP) mRNA and protein was investigated in the olfactory mucosa of humans ranging in age from 26 weeks of gestation to 85 years using in situ hybridization and immunocytochemistry. OMP mRNA and protein were most abundant in the somas of olfactory receptor neurons (ORNs). The hybridization signal over the ORN somal layer was greater in older subjects than in younger ones, reflecting either a higher neuronal density or more OMP mRNA per cell. In contrast, it was significantly lower in subjects with Alzheimer's disease when compared with an age-matched control. Characteristics of older subjects were patchiness in the distribution of OMP-expressing ORNs and the occurrence of subepithelial invaginations containing OMP-positive neurons. In addition, a significant hybridization signal was detected in the apical olfactory epithelium containing the dendrites, dendritic knobs, and cilia of ORNS, and over olfactory nerve bundles in the lamina propria, indicating the occurrence of OMP mRNA in dendritic and axonal domains.