MEASURES OF LEUCINE AMINOPEPTIDASE CAN BE USED TO ANTICIPATE UV-INDUCED AGE-RELATED DAMAGE TO LENS PROTEINS - ASCORBATE CAN DELAY THIS DAMAGE
MEASURES OF LEUCINE AMINOPEPTIDASE CAN BE USED TO ANTICIPATE UV-INDUCED AGE-RELATED DAMAGE TO LENS PROTEINS - ASCORBATE CAN DELAY THIS DAMAGE
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DOI：
10.1016/0047-6374(87)90052-2
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发表时间：
1987-11-01
影响因子：
5.3
通讯作者：
TAYLOR, A
中科院分区：
文献类型：
--
作者：
BLONDIN, J;TAYLOR, A
本文着重介绍紫外线（UV）照射对可溶性透镜蛋白的损伤及抗坏血酸（维生素C）的预防作用。使用2.3 ×10-3 W/cm 2 UV A和0.4 × UV A。10-4 W/cm 2紫外线B，氨肽酶失活透镜上清液是显着的60分钟后。蛋白质聚集和色氨酸水平的下降，与紫外线诱导和白内障相关的损害的现象，观察到只有在较长的（6小时）紫外线暴露。因此，似乎氨肽酶活性的测量可用于预测对透镜结构蛋白的损伤。抗坏血酸（15 mM）加入到可溶性透镜蛋白之前，光照射可以防止这些变化。所提供的数据表明蛋白水解受损和白内障形成之间的合理关系。
This paper focuses on damage to soluble lens proteins during ultraviolet (UV) light exposure and its prevention by ascorbate (Vitamin C). Using 2.3 .times. 10-3 W/cm2 UV A and 0.4 .times. 10-4 W/cm2 UV B, aminopeptidase inactivation in lens supernatants is significant after 60 min. Protein aggregation and decreases in tryptophan levels, phenomena associated with UV-induced and cataract-related damage, are observed only after longer (6 h) UV exposure. Thus, it would appear that measurements of aminopeptidase activity can be used to anticipate damage to lens structural proteins. Ascorbate (15 mM) added to soluble lens proteins prior to photoirradiation can prevent some of these changes. The data presented suggest plausible relationships between impaired proteolysis and cataract formation.