Layer by layer self-assembly for coating a nanosuspension to modify drug release and stability for oral delivery
Layer by layer self-assembly for coating a nanosuspension to modify drug release and stability for oral delivery
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DOI：
10.1016/j.foodhyd.2023.108908
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发表时间：
2023-05
影响因子：
10.7
通讯作者：
Nancy M. Elbaz;Lee M. Tatham;A. Owen;S. Rannard;Tom O. McDonald
中科院分区：
文献类型：
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作者：
Nancy M. Elbaz;Lee M. Tatham;A. Owen;S. Rannard;Tom O. McDonald
逐层修饰是一种有效的调整粒子性质的方法。然而，传统的LbL工艺涉及重复的洗涤步骤，这与具有部分溶解度的纳米颗粒不兼容。在这项工作中，我们展示了使用滴定法在姜黄素纳米悬浮液（一种具有有限水溶性的化合物）上生产LbL涂层。这项工作的目的是展示如何使用LbL来增强姜黄素在纳米悬浮液中的释放行为和稳定性。包被的纳米悬浮液样品由生物相容性和可生物降解的聚电解质、聚l-精氨酸和海藻酸钠制成，尺寸约为500 nm。采用聚l-精氨酸和海藻酸盐包覆5层纳米悬浮液，最外层为乌龙籽油L100，制备了一种刺激响应型纳米悬浮液。这些纳米混悬液的体外释放表明，使用pH响应层（Eudragit L100）作为最外层导致姜黄素在酸性pH下的释放延迟（5%），并促进其在中性pH下的释放（11%）超过72小时。生物可及性研究表明，当暴露于模拟胃肠道的条件下，增加层数导致纳米混悬液中姜黄素的稳定性从未涂覆纳米混悬液的20%增加到4层涂覆纳米混悬液的40%和90%，刺激响应（6层）涂覆纳米混悬液。纳米混悬液的细胞毒性显示姜黄素的毒性降低。这项工作展示了如何使用滴定LbL修饰方法来定制用于口服药物递送应用的纳米混悬液的稳定性和释放行为。
Layer-by-layer (LbL) modification is an effective way to tune the properties of particles. However, the traditional LbL process involves repeated washing steps which are not compatible with nanoparticles with a partial solubility. In this work, we demonstrate the use of a titration method for producing LbL coatings onto a nanosuspension of curcumin (a compound with a limited aqueous solubility). The aim of the work was to show how LbL can be used to enhance the release behaviour and stability of the curcumin in a nanosuspension form. Coated nanosuspension samples were produced using biocompatible and biodegradable polyelectrolytes, poly-l-arginine, and sodium alginate and was sizes approximately 500 nm. A stimuli-responsive nanosuspension was prepared by coating the nanosuspension with 5 layers of poly-l-arginine and alginate and Eudragit L100 as an outmost layer. Thein vitrorelease of these nanosuspensions revealed that the use of a pH-responsive layer (Eudragit L100) as an outermost shell resulted in delay the release of curcumin (5%) in acidic pH and facilities its release in neutral pH (11%) over 72 h. Additionally, the bioaccessibility study showed that increasing the number of layers resulted in increasing the stability curcumin in nanosuspension when exposed to conditions that mimic the gastrointestinal tract from 20% for uncoated nanosuspension to 40% and 90% for 4-layered coated nanosuspension and stimuli-responsive (6-layered) coated nanosuspension, respectively. The cytotoxicity of LbL-coating of nanosuspension revealed a reduction in the toxicity of curcumin. This work shows how a titrated LbL modification approach could be used to tailor the stability and the release behaviour of nanosuspensions for oral drug delivery applications.