Sensitive colorimetric assay for angiotensin converting enzyme in serum.
Sensitive colorimetric assay for angiotensin converting enzyme in serum.
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DOI：
10.1093/clinchem/29.7.1399
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发表时间：
1983-07
影响因子：
9.3
通讯作者：
H. Neels;M. van Sande;S. Scharpe
中科院分区：
文献类型：
--
作者：
H. Neels;M. van Sande;S. Scharpe
建立了一种灵敏的比色法测定血清中血管紧张素转换酶（EC 3.4.15.1）。如前所述（Clin Chem 28：1352-1355，1982），将血清（10 μ L）与马尿酰-甘氨酰-甘氨酸孵育30分钟。Folin-Wu脱蛋白后，用硼酸盐缓冲（pH 9.3）三硝基苯磺酸盐溶液（60 mmol/L）衍生释放的甘氨酰-甘氨酸，形成三硝基苯基-甘氨酰甘氨酸，在420 nm处相对于血清空白读取其吸光度。线性范围可达900 U/L以上，检出限小于4 U/L。50名血库捐献者和25名活动性结节病患者血清的平均活性分别为281（SD 77）和693（SD 81）U/L。该方法表现出良好的精密度（CV小于2.8%）和相关性以及（r = 0.99）从“高压”液相色谱法测定马尿酸的结果。此外，所提出的方法是广泛适用的，只涉及常用的设备。
A sensitive colorimetric procedure has been developed for the assay of angiotensin converting enzyme (EC 3.4.15.1) in serum. Serum (10 microL) is incubated for 30 min with hippuryl-glycyl-glycine as described earlier (Clin Chem 28: 1352-1355, 1982). After a Folin-Wu deproteinization, the liberated glycyl-glycine is derivatized with a borate-buffered (pH 9.3) trinitrobenzenesulfonate solution (60 mmol/L) to form trinitrophenyl-glycylglycine, the absorbance of which is read at 420 nm vs a serum blank. The linear range extends to an activity of more than 900 U/L of serum and the detection limit is less than 4 U/L. The mean activity for serum from 50 blood bank donors and 25 patients with active sarcoidosis was 281 (SD 77) and 693 (SD 81) U/L, respectively. The method demonstrates good precision (CVs less than 2.8%) and correlates well (r = .99) with results from a "high-pressure" liquid chromatographic procedure for determining hippuric acid. In addition, the proposed method is widely applicable, involving only commonly available apparatus.