Diel transcriptional response of a California Current plankton microbiome to light, low iron, and enduring viral infection
Diel transcriptional response of a California Current plankton microbiome to light, low iron, and enduring viral infection
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DOI：
10.1038/s41396-019-0472-2
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发表时间：
2019-11-01
期刊：
影响因子：
11
通讯作者：
Allen, A. E.
中科院分区：
文献类型：
--
作者：
Kolody, B. C.;McCrow, J. P.;Allen, A. E.
浮游植物和相关的微生物群落为海洋食物网提供有机碳，并驱动生态系统动态。然而，捕捉这些动态是具有挑战性的。在这里，一个在原位，半拉格朗日，机器人采样器剖析浮游微生物在4小时的时间间隔超过类似的2.6天，在北太平洋高营养，低叶绿素沃茨。我们报告的社区结构和转录动力学的微生物在一个操作的大尺寸类（>5 μ m）主要由甲藻，纤毛虫，附着，pelagophytes，硅藻，蓝藻（主要是聚球藻），prasinophytes（主要是Ostreococcus），真菌，古菌和变形菌。除了真菌和古细菌，所有群体都表现出24小时的周期性在一些成绩单，但大部分的转录组振荡的光养生物。周期性光合作用相关的成绩单表现出时间级联整个上午的时间，保存在不同的光养谱系。显著的二氧化硅：硝酸盐下降，高黄素氧还蛋白铁氧还蛋白转录比，和其他铁胁迫标志物的表达升高表明铁限制。铁胁迫标志物在光周期适应时间窗口，可以调节浮游植物响应季节性铁限制达到峰值。值得注意的是，我们观察到的病毒感染大多数丰富的类群，往往与推定的主机同步的总转录活性。总之，这些数据揭示了一个浮游微生物群落，是由回收生产和严格控制的铁限制和病毒活性。
Phytoplankton and associated microbial communities provide organic carbon to oceanic food webs and drive ecosystem dynamics. However, capturing those dynamics is challenging. Here, an in situ, semi-Lagrangian, robotic sampler profiled pelagic microbes at 4 h intervals over similar to 2.6 days in North Pacific high-nutrient, low-chlorophyll waters. We report on the community structure and transcriptional dynamics of microbes in an operationally large size class (>5 mu m) predominantly populated by dinoflagellates, ciliates, haptophytes, pelagophytes, diatoms, cyanobacteria (chiefly Synechococcus), prasinophytes (chiefly Ostreococcus), fungi, archaea, and proteobacteria. Apart from fungi and archaea, all groups exhibited 24-h periodicity in some transcripts, but larger portions of the transcriptome oscillated in phototrophs. Periodic photosynthesis-related transcripts exhibited a temporal cascade across the morning hours, conserved across diverse phototrophic lineages. Pronounced silica:nitrate drawdown, a high flavodoxin to ferredoxin transcript ratio, and elevated expression of other Fe-stress markers indicated Fe-limitation. Fe-stress markers peaked during a photoperiodically adaptive time window that could modulate phytoplankton response to seasonal Fe-limitation. Remarkably, we observed viruses that infect the majority of abundant taxa, often with total transcriptional activity synchronized with putative hosts. Taken together, these data reveal a microbial plankton community that is shaped by recycled production and tightly controlled by Fe-limitation and viral activity.