Metabolomic profiling of brassinolide and abscisic acid in response to high-temperature stress
Metabolomic profiling of brassinolide and abscisic acid in response to high-temperature stress
复制标题
油菜素内酯和脱落酸响应高温胁迫的代谢组学分析
DOI：
10.1007/s00299-022-02829-2
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发表时间：
2022-01-19
影响因子：
6.2
通讯作者：
Fang, Jinggui
中科院分区：
文献类型：
--
作者：
Lv, Jinhua;Dong, Tianyu;Fang, Jinggui
激素处理提高了高温条件下渗透物质的含量。阿坝和BR单独处理的效果优于联合处理。为研究脱落酸（阿坝）、芸苔素（BR）及阿坝+ BR对高温胁迫下葡萄果实品质的影响，分析了高温胁迫下ABA、BR和ABA对葡萄果实品质的影响。与对照相比，阿坝处理后DL-色氨酸、D-棉子糖、京尼平苷酸、十二烷二酸和多酚类物质含量均有所增加。BR处理后，氨基酸和尿酸B含量均高于对照。阿坝+ BR处理后，碳水化合物和氨基酸含量均显著增加。BR和阿坝+ BR处理也诱导了较高的内源阿坝和表油菜素含量。此外，处理葡萄的可溶性固形物含量和可溶性糖含量较高，延缓了中片层和微纤丝的降解。抗氧化和热休克相关基因进行了检测，显着增加处理的葡萄。本研究结果表明，阿坝、BR和阿坝+ BR通过增加渗透调节物质的积累和内源激素含量，对缓解高温伤害非常有用。
Key message Hormone treatment enhanced the content of osmotic substances under high-temperature conditions. The effect of ABA and BR treated separately is better than treated together. To determine the effect of abscisic acid (ABA), brassinolide (BR) and ABA + BR on grape quality under high-temperature stress, various metabolites were analyzed. Compared with the control (CK), DL-tryptophan, d-raffinose, geniposidic acid, dodecanedioic acid and polyphenols were found to be higher after ABA treatment. After BR treatment, amino acids and poricoic acid B were higher than in CK. And carbohydrates and amino acids were up-regulated after ABA + BR treatment. BR and ABA + BR treatment also induced higher endogenous ABA and epibrassinolide contents. In addition, treated grape had higher soluble solid concentrations and soluble sugar content, and delayed the degradation of middle lamella and microfibrils. Antioxidant and heat shock-related genes were examined, which significantly increased in treated grape. The finding of this study suggested that ABA, BR and ABA + BR are very useful for alleviating high-temperature damage by increasing the accumulation of osmotic adjustment substances, and endogenous hormones content.