Optimizing microenvironment by integrating negative pressure and exogenous electric fields via a flexible porous conductive dressing to accelerate wound healing
Optimizing microenvironment by integrating negative pressure and exogenous electric fields via a flexible porous conductive dressing to accelerate wound healing
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通过柔性多孔导电敷料整合负压和外源电场来优化微环境，加速伤口愈合
DOI：
10.1039/d0bm01172j
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发表时间：
2021-01-07
影响因子：
6.6
通讯作者：
Zhang, Jiaping
中科院分区：
文献类型：
--
作者：
Chen, Ying;Liang, Yi;Zhang, Jiaping
伤口愈合是一个复杂的、循序渐进的生物过程，涉及多个阶段。目前对无法愈合或慢性伤口的治疗方法并不令人满意，因为它们只对一种特定的活动产生单一的影响。在这里，我们构建了一种基于银纳米线(AgNW)的三维(3D)多孔泡沫敷料，这种敷料具有柔性和导电性。这种导电泡沫敷料由稳定的疏水涂层改性的AgNW和支持3D导电网络的多孔聚氨酯(PU)组成。AgNWs-PU泡沫敷料具有良好的生物相容性、优异的电学性能、优异的弯曲-压缩耐久性以及在潮湿条件下的长期稳定性，适合于伤口治疗。通过导电性泡沫敷料，负压和外源伤口定向电场(EFS)可以集成在一起同时实施，人工共同构建的微环境在一个系统中促进伤口愈合。这种新型的“一体式”设备具有内在的多功能，包括引流脓液和坏死组织、减轻炎症、促进细胞增殖、引导角质形成细胞迁移和诱导血管生成。免疫组织化学分析和免疫印迹分析表明，当采用这种新的治疗方法时，组织中的血管生成和细胞增殖途径被显著激活。更重要的是，通过对模型猪全层创面的评估，证明了这种“一体式”装置的实用性能。比较传统疗法和新疗法的残余创面面积百分比(25.82+/-3.52%和3.07+/-1.23%)，表明这种新疗法在临床创面愈合中具有广阔的应用前景。
Wound healing is a complex and sequential biological process that involves multiple stages. Current treatments for nonhealing or chronic wounds are unsatisfactory as they exert a single effect on one specific activity. Herein, we constructed a silver nanowire (AgNW)-based, three-dimensional (3D), porous foam dressing that is flexible and conductive. This conductive foam dressing was composed of AgNWs modified with a stable hydrophobic coating and porous polyurethane (PU), providing a skeleton to support the 3D conductive networks. The AgNWs-PU foam dressing exhibited favorable biocompatibility, outstanding electrical properties, excellent bending-compression durability, and long-term stability under wet conditions, making it suitable for wound treatment. Via the conductive foam dressing, negative pressure and exogenous wound directional electric fields (EFs) could be integrated for simultaneous implementation, and the artificial jointly constructed microenvironment promoted wound healing in a system. This novel "all-in-one" device presented intrinsic multifunctionality, including the drainage of pus and necrotic tissue, mitigation of inflammation, promotion of cell proliferation, direction of keratinocyte migration, and induction of angiogenesis. An immunohistochemical assay and western blot analysis illustrated that the angiogenesis and cell proliferation pathways in the tissue were significantly activated when this novel therapy was adopted. More importantly, the practical performance of this "all-in-one" device was demonstrated by assessment of full-thickness defect wounds in model pigs. Comparing the percentage of residual wound area after administration of traditional treatment (25.82 +/- 3.52%) and the novel treatment (3.07 +/- 1.23%) demonstrated the promising applications of this novel treatment in clinical wound healing.