Functional CCR9 expression is associated with small intestinal metastasis
Functional CCR9 expression is associated with small intestinal metastasis
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DOI：
10.1111/j.0022-202x.2004.22315.x
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发表时间：
2004-03-01
影响因子：
6.5
通讯作者：
Scheibenbogen, C
中科院分区：
文献类型：
--
作者：
Letsch, A;Keilholz, U;Scheibenbogen, C
一般来说，转移到小肠是罕见的，主要发生在黑色素瘤。CCR 9已被证明是胸腺表达趋化因子（TECK）的主要趋化因子受体，TECK是在小肠和胸腺中选择性表达的趋化因子。在这里，我们表明，CCR 9是高度表达的黑色素瘤细胞和所有的黑色素瘤细胞系分离的小肠转移，并在一定比例的细胞系从其他网站。然而，只有黑色素瘤细胞和小肠转移瘤细胞系对CCR 9配体TECK有反应，这通过受体下调和肌动蛋白聚合来评估。在表达肠细胞分化特征的腺癌细胞系CaCo-2上也发现了CCR 9表达，但在从结直肠癌、乳腺癌和肺癌分离的任何其他细胞系上没有发现CCR 9表达。我们的数据提供的证据表明，异常的功能细胞表面表达的器官特异性趋化因子受体与转移到这个网站。受体功能的调节似乎是转移过程中的关键步骤。
In general, metastases to the small intestine are rare, and mostly occur in melanoma. CCR9 has been shown to be the principal chemokine receptor for the thymus expressed chemokine (TECK), a chemokine selectively expressed in the small intestine and thymus. Here we show that CCR9 is highly expressed on melanoma cells and all melanoma cell lines isolated from small intestinal metastases, and on a proportion of cell lines from other sites. Only melanoma cells and cell lines from small intestinal metastases, however, were responsive to the CCR9 ligand TECK, as assessed by receptor downregulation and by actin polymerization. CCR9 expression was also found on the adenocarcinoma cell line CaCo-2 expressing characteristics of enterocytic differentiation, but not on any other cell line isolated from colorectal, breast, and lung cancer. Our data provide evidence that the aberrant functional cell surface expression of an organ-specific chemokine receptor is associated with metastasis to this site. The regulation of receptor function seems to be a critical step in the metastatic process.