Dual regulation of insulin-like growth factor I expression during renal hypertrophy.
Dual regulation of insulin-like growth factor I expression during renal hypertrophy.
复制标题
肾肥大期间胰岛素样生长因子 I 表达的双重调节。
DOI：
10.1152/ajprenal.1989.257.2.f252
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Hammerman,MR
中科院分区：
文献类型：
--
作者：
Lajara,R;Rotwein,P;Bortz,JD;Hansen,VA;Sadow,JL;Betts,CR;Rogers,SA;Hammerman,MR
我们研究了两种不同实验模型产生的肾脏肥大过程中肾脏中胰岛素样生长因子I（IGF-I）的调节：垂体切除大鼠的生长激素治疗和单侧肾切除后的代偿性肥大。生长激素缺乏的垂体切除大鼠肾脏集合管IGF-I免疫染色增强，IGF-I mRNA水平升高。在代偿性肥大，没有增强的免疫染色的强度，观察到肾切除大鼠的肾脏，直到5天postnephrectomy，在这个时候，免疫组织化学IGF-I显着增加，在髓集合管肥大的肾脏相比，从假手术动物的肾脏。在肾切除术后的任何时间，在整个肾脏或肾切除或假手术大鼠的集合管中检测到任何IGF-I mRNA的稳态水平没有差异。我们的研究结果表明，IGF-I mRNA和IGF-I在集合管中的生长激素诱导的肾脏肥大的设置增加，但表明，其他的，可能是翻译，机制的基础上诱导IGF-I合成在代偿性肥大。
We examined the regulation of insulin-like growth factor I (IGF-I) in kidney during the renal hypertrophy produced by two different experimental models: growth hormone treatment of hypophysectomized rats and compensatory hypertrophy subsequent to unilateral nephrectomy. Immunostaining for IGF-I in collecting ducts was enhanced in kidneys from growth hormone-repleted hypophysectomized rats, and the levels of IGF-I mRNAs were increased. In compensatory hypertrophy, no enhancement of the intensity of immunostaining was observed in kidneys of nephrectomized rats until 5 days postnephrectomy, at which time immunostainable IGF-I was increased markedly in medullary collecting ducts of hypertrophied kidneys compared with kidneys from sham-operated animals. No difference in steady-state levels of any IGF-I mRNA species was detected in whole kidneys or in collecting ducts from nephrectomized or sham-operated rats at any time postnephrectomy. Our findings demonstrate an increase in both IGF-I mRNA and in immunostainable IGF-I in collecting duct in the setting of growth hormone-induced renal hypertrophy but suggest that other, possibly translational, mechanisms underlie the induction of IGF-I synthesis during compensatory hypertrophy.