Fine structural analysis of the epithelial cells in the hepatopancreas of Palaemonetes argentinus (Crustacea, Decapoda, Caridea) in intermoult.
Fine structural analysis of the epithelial cells in the hepatopancreas of Palaemonetes argentinus (Crustacea, Decapoda, Caridea) in intermoult.
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DOI：
10.32604/biocell.2005.29.025
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发表时间：
2005-04
期刊：
影响因子：
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通讯作者：
L. Sousa;E. I. Cuartas;A. Petriella
中科院分区：
文献类型：
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作者：
L. Sousa;E. I. Cuartas;A. Petriella
本文研究了蜕皮间期阿根廷对虾肝胰腺的超微结构。使用标准TEM技术研究阿根廷对虾肝胰腺。每个小管由四种细胞类型组成：E（胚胎）、F（纤维状）、R（吸收性）和B（水泡状）。E细胞具有胚胎特征，其中一些细胞在有丝分裂中被发现。F、R和B细胞具有顶端刷状缘。F细胞有一个中央或基底核，一个明显的粗面内质网，和扩张的高尔基池。R细胞在三个区域中显示出细胞器的极性组织：顶端，具有许多线粒体和sER小管，具有细胞核和粗面内质网的中心区域，以及含有sER样小管系统和线粒体的基底区域。在其生命周期的不同阶段观察B细胞。在早期分化阶段，它们包括顶端内吞复合体和高尔基体小泡。内吞囊泡和高尔基体囊泡的融合形成近顶端的液泡。在成熟过程中，一个大的中央液泡是由近顶端的液泡合并而成的。中央空泡由全分泌分泌消除。超微结构表明，F细胞具有合成蛋白质的功能，R细胞具有贮存营养的功能，B细胞具有分泌或排泄的功能，证实了F、B和R细胞均独立起源于胚胎细胞。
The aim of this study is to describe the ultrastructure of the hepatopancreas of P. argentinus in intermoult. P. argentinus hepatopancreas was studied using standard TEM techniques. Each tubule consists of four cellular types: E (embryonic), F (fibrillar), R (resorptive) and B (blister like). E-cells have embryonic features and some of them were found in mitosis. F, R and B cells possess an apical brush border. F-cells have a central or basal nucleus, a conspicuous RER, and dilated Golgi cisternae. R cells show a polar organization of organelles in three areas: apical, with numerous mitochondria and sER tubules, a central area with the nucleus and RER, and a basal area containing a sER-like tubule system and mitochondria. B-cells were observed at different stages of their life cycle. In an early differentiation stage they comprise an apical endocytotic complex and Golgi vesicles. The fusion of endocytotic and Golgi vesicles originates subapical vacuoles. During maturation, a big central vacuole is formed by coalescence of subapical vacuoles. The central vacuole is eliminated by holocrine secretion. The ultrastructure suggests that F-cells synthesize proteins, R-cells storage nutrients and B-cells have a secretory or excretory function, and confirms the independent origin of F, B and R cells from the embryonic cells.