A TWO-DIMENSIONAL ELECTROPHORETIC TECHNIQUE FOR THE DETECTION OF CIRCULAR VIROIDS AND VIRUSOIDS
A TWO-DIMENSIONAL ELECTROPHORETIC TECHNIQUE FOR THE DETECTION OF CIRCULAR VIROIDS AND VIRUSOIDS
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DOI：
10.1016/0003-2697(83)90685-1
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发表时间：
1983-01-01
影响因子：
2.9
通讯作者：
RIESNER, D
中科院分区：
文献类型：
--
作者：
SCHUMACHER, J;RANDLES, JW;RIESNER, D
本文介绍了一种新的凝胶电泳技术，用于快速、灵敏地检测环状类病毒和类病毒。从植物[番茄]材料开始，典型的多样品分析需要< 8小时。在不使用放射性或高度专业化的实验室设备的情况下，可以明确地检测到低至60纳克/克组织的类病毒浓度。这里提出的技术相比，凝胶电泳，核酸指纹图谱，目前采用的杂交技术的早期方法。许多重要的技术优势，包括速度、简单性和灵敏度，表明本文描述的方法在检查类病毒感染的传播方面可能具有广泛的实用性。
A new gel electrophoretic technique for the rapid and sensitive detection of circular viroids and virusoids is described. Starting from plant [tomato] material, a typical multisample analysis requires < 8 h. Viroid concentrations as low as 60 ng/g tissue can be detected unambiguously without the use of radioactivity or highly specialized laboratory equipment. The technique presented here is compared to earlier methods of gel electrophoresis, nucleic acid fingerprinting, and currently employed hybridization techniques. A number of important technical advantages, including speed, simplicity, and sensitivity, suggest that the methods described here may have wide utility in checking the spread of viroid infections.