Expression of SULT1E1 protein in bovine placentomes: Evidence for localization in uninucleated trophoblast cells
Expression of SULT1E1 protein in bovine placentomes: Evidence for localization in uninucleated trophoblast cells
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DOI：
10.1016/j.placenta.2011.03.002
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发表时间：
2011-06-01
期刊：
影响因子：
3.8
通讯作者：
Schuler, G.
中科院分区：
文献类型：
--
作者：
Khatri, P.;Frenette, G.;Schuler, G.
牛胎盘产生大量与妊娠相关的雌激素，主要是硫酸雌酮，它不与经典的核雌激素受体相互作用。因此，牛胎盘雌激素可能更像是局部调节因子，而不是经典意义上的激素。为了了解牛胎盘中游离雌激素的局部可获得性，分别用抗牛酶和人酶的抗血清研究了雌激素特异性磺基转移酶SULT1E1的表达模式。在蛋白质印迹中，两种抗血清都检测到一条预期分子量的条带(约.胎盘和胎肝中均为33 kDa。在免疫组织化学中，两种抗血清产生了几乎相同的结果。在胎盘中，仅在单核滋养层细胞(UTC)的胞浆中有明显染色。在UTC、未成熟和成熟滋养层巨细胞(TGC)中的染色模式与TGC分化过程中SULT1E1的下调一致。在妊娠早期和中期，UTC在覆盖绒毛板和茎绒毛基底部的滋养层中的染色强度高于绒毛树的其他区域，而在妊娠晚期和分娩时，UTC的染色几乎无处不在。相应地，实时定量RT-PCR法测定的SULT1E1mRNA的相对水平在妊娠晚期显著增加(p=0.0043)。与胎盘和胎肝相比，成年牛器官中SULT1E1-mRNA的表达水平显著降低。结果表明，在UTC和胎肝中表达的SULT1E1抑制了牛TGC产生的游离雌激素的活性。滋养层巨细胞产生的与妊娠相关的雌激素主要在单核滋养层细胞中磺化。(C)2011爱思唯尔有限公司。保留所有权利。
The bovine placenta produces considerable amounts of pregnancy-associated estrogens, predominantly estrone sulfate, which does not interact with classical nuclear estrogen receptors. Thus, bovine placental estrogens may rather act as local regulatory factors than as hormones in a classical sense. To obtain information on the local availability of free estrogens in bovine placentomes, the expression pattern of the estrogen specific sulfotransferase SULT1E1 was characterized using antisera against the bovine and human enzyme, respectively. In western blot both antisera detected a band of the expected molecular weight (approx. 33 kDa) in placentomes and fetal liver. In immunohistochemistry the two antisera yielded virtually identical results. In placentomes distinct staining was restricted to the cytosol of uninucleated trophoblast cells (UTC). The staining pattern in UTC, immature and mature trophoblast giant cells (TGC) is consistent with a down-regulation of SULT1E1 during TGC differentiation. During early and midgestation staining intensity in UTC was higher in the trophoblast covering the chorionic plate and basal parts of stem villi compared to other regions of the villous tree, whereas during late gestation and at parturition an almost ubiquitous distinct staining of UTC was found. Correspondingly, relative SULT1E1-mRNA levels as measured by quantitative real-time RT-PCR increased significantly during late gestation (p = 0.0043). Comparative measurements showed that SULT1E1-mRNA levels in adult bovine organs were considerably lower compared to placentomes and fetal liver. The results suggest that the activities of free estrogens produced in bovine TGC are curtailed by SULT1E1 expressed in UTC and in fetal liver.Bovine pregnancy-associated estrogens produced in trophoblast giant cells are predominantly sulfonated in uninucleated trophoblast cells. (C) 2011 Elsevier Ltd. All rights reserved.