Successful mouse cloning of an outbred strain by trichostatin A treatment after somatic nuclear transfer
Successful mouse cloning of an outbred strain by trichostatin A treatment after somatic nuclear transfer
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DOI：
10.1262/jrd.18098
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发表时间：
2007-02-01
影响因子：
1.8
通讯作者：
Wakayama, Teruhiko
中科院分区：
文献类型：
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作者：
Kishigami, Satoshi;Bui, Hong-Thuy;Wakayama, Teruhiko
尽管体细胞克隆技术已被用于各种物种重编程的大量应用和基础研究，但十年来这种技术的成功率极低。在小鼠中，“可克隆”的菌株主要局限于杂交F1菌株，如B6D2F1。最近，我们建立了一种利用曲古霉素a （trichostatin a， TSA）高效克隆小鼠B6D2F1积云细胞的新技术，使其克隆成功率提高了2-5倍。为了进一步验证这种TSA克隆技术的有效性，我们尝试克隆一种以前从未被直接克隆过的近交系成年ICR小鼠。只有在使用TSA时，我们才从成年ICR小鼠的积云细胞和成纤维细胞中获得雄性和雌性克隆小鼠，成功率为4-5%，与B6D2F1的5-7%相当。因此，TSA处理是第一个使我们能够成功克隆远交小鼠的克隆技术，这表明该技术不仅提高了杂交菌株克隆的成功率，而且使小鼠能够从通常“不可克隆”的菌株中克隆。
Although the somatic cloning technique has been used for numerous applications and basic research of reprogramming in various species, extremely low success rates have plagued this technique for a decade. Further in mice, the "clonable" strains have been limited to mainly hybrid F1 strains such as B6D2F1. Recently, we established a new efficient cloning technique using trichostatin A (TSA) which leads to a 2-5 fold increase in success rates for mouse cloning of B6D2F1 cumulus cells. To further test the validity of this TSA cloning technique, we tried to clone the adult ICR mouse, an outbred strain, which has never been directly cloned before. Only when TSA was used did we obtain both male and female cloned mice from cumulus and fibroblast cells of adult ICR mice with 4-5% success rates, which is comparable to 5-7% of B6D2F1. Thus, the TSA treatment is the first cloning technique to allow us to successfully clone outbred mice, demonstrating that this technique not only improves the success rates of cloning from hybrid strains, but also enables mouse cloning from normally "unclonable" strains.