Molecularly Imprinted Solid Phase Extraction using Bismethacryloyl-β-cyclodextrin and Methacrylic Acid as Double Functional Monomers for Selective Analysis of Glycyrrhizic Acid in Aqueous Media
Molecularly Imprinted Solid Phase Extraction using Bismethacryloyl-β-cyclodextrin and Methacrylic Acid as Double Functional Monomers for Selective Analysis of Glycyrrhizic Acid in Aqueous Media
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DOI：
10.1093/chromsci/bmw161
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发表时间：
2017-02-01
影响因子：
1.3
通讯作者：
Fu, Qiang
中科院分区：
文献类型：
--
作者：
Tang, Weili;Du, Wei;Fu, Qiang
本工作建立了一种新的分子印迹固相萃取方法，用于水介质中选择性提取和纯化甘草中的甘草酸。以双甲基丙烯酰-β-环糊精和甲基丙烯酸为双功能单体，制备了辛酸的分子印迹聚合物（MIPs），并采用傅里叶变换红外光谱、扫描电镜、热重分析、氮吸附和元素分析等方法对MIPs进行了表征。在水介质中，研究了分子印迹聚合物的吸附动力学、吸附等温线和选择性吸附。对印迹聚合物的表征表明，所制备的印迹聚合物具有良好的稳定性和多空腔结构。吸附实验结果表明，该分子印迹聚合物对草酸的吸附容量为69.3mg g（-1），印迹因子为3.77，吸附平衡时间约为5 min。该分子印迹聚合物可作为固相萃取吸附剂富集纯化甘草粗提物中的甘草酸，具有较好的实用价值。
In this work, a new molecularly imprinted solid phase extraction protocol was developed for the selective extraction and purification of glycyrrhizic acid from liquorice roots in aqueous media. The molecularly imprinted polymers (MIPs) for glycyrrhizic acid were prepared by using bismethacryloyl-beta-cyclodextrin and methacrylic acid as double functional monomers and characterized by Fourier transform infrared spectroscopy, scanning electron microscope, thermo gravimetric analysis, nitrogen adsorption and elemental analysis. In aqueous media, the adsorption properties of MIPs including adsorption kinetics, adsorption isotherms and selectivity adsorption were investigated. The characterization of imprinted polymers indicated that the prepared MIPs had good stability and many cavity structures. The results of adsorption experiments illustrated the MIPs had high adsorption capacity of glycyrrhizic acid (69.3 mg g(-1)) with the imprinting factor 3.77, and it took similar to 5 min to get adsorption equilibrium. The MIPs could be used as an solid phase extraction sorbent absorbent for enrichment and purification of glycyrrhizic acid from the crude extraction of licorice roots, and the results showed promising practical value.